Yazhangsan (YZS) is a common prescription for the treatment of cough and asthma caused by wind‐cold. The purpose of this study was to investigate the pharmacokinetic profiles of 10 bioactive components in YZS. A simple, sensitive and reliable high‐performance liquid chromatography coupled with a triple‐quadruple mass spectrometry method (LC–MS/MS) was developed and fully validated in this study for the measurement of these 10 bioactive compounds in rat plasma. One‐step protein precipitation method using methanol was applied to the treatment of rat plasma samples. Chromatographic separation was conducted on a C₁₈ column by gradient elution, and water (containing 0.1% formic acid) and acetonitrile were chosen as the mobile phase. The analytes were quantified by using a mass spectrometer in multiple reaction monitoring scanning mode, and electrospray ionization was performed in positive and negative ion modes. The established method met the requirements for the quantification of these 10 bioactive compounds in biological samples, and it was successfully applied to the pharmacokinetic study of 10 components in rats after the intragastrical administration of YZS. This study will lay a foundation for the investigation of the mechanism of action of YZS and provide useful data for the rational use of YZS in clinical.

中文翻译：

---

LC-MS / MS法定量测定大鼠血浆中Yazhangsan的10种生物活性成分及其应用。

Yazhangsan（YZS）是治疗风寒引起的咳嗽和哮喘的常用处方。这项研究的目的是调查YZS中10种生物活性成分的药代动力学特征。开发了一种简单，灵敏，可靠的高效液相色谱法，并结合了三重四重质谱法（LC-MS / MS），并在本研究中进行了充分验证，可用于测定大鼠血浆中的这10种生物活性化合物。使用甲醇的一步蛋白质沉淀法被用于处理大鼠血浆样品。色谱分离是在C _{18上进行的}通过梯度洗脱柱分离，并选择水（含有0.1％的甲酸）和乙腈作为流动相。通过使用质谱仪在多反应监测扫描模式下对分析物进行定量，并在正离子和负离子模式下进行电喷雾电离。建立的方法满足了对生物样品中这10种生物活性化合物进行定量的要求，并将其成功地用于YZS胃内给药后大鼠中10种成分的药代动力学研究。该研究将为研究YZS的作用机理奠定基础，并为临床合理使用YZS提供有用的数据。