This study explored molecular mechanisms of resveratrol (RES) on regulating skeletal fiber-type switching in pigs. RES had no effect on growth performance of pigs. Remarkably, myosin heavy chain (MyHC)1 and MyHC2a mRNA level were increased, while MyHC2b decreasing in Longissimus thoracis (LT) after RES treatment. Meanwhile, RES increased enzyme activities including Succinate Dehydrogenase (SDH) and Malate Dehydrogenase (MDH) in LT. Moreover, RES upregulated the plasma AdipoQ concentration, the expression of AdipoQ, Adiponectin receptor 1(AdipoR1), AdipoR2, AMPK and PGC-1α in AdipoQ signaling pathway in pigs. In addition, RES treating the porcine skeletal muscle satellite cells results showed that RES increased the expression of AdipoR1, AMPK, PGC-1α, MyHC1 and MyHC2a, while decreasing MyHC2b expression. Although RES was added into cells, the AMPK and PGC-1α expression could not be increased by knockdown of AdipoR1 expression. This study demonstrated that RES increased the proportion of oxidative muscle fiber through the AdipoR1-AMPK-PGC-1α pathway in pigs.

这项研究探讨了白藜芦醇（RES）调节猪骨骼纤维类型转换的分子机制。RES对猪的生长性能没有影响。值得注意的是，经RES处理后，在胸大肌（LT）中，肌球蛋白重链（MyHC）1和MyHC2a mRNA水平升高，而MyHC2b下降。同时，RES增加了LT中的酶活性，包括琥珀酸脱氢酶（SDH）和苹果酸脱氢酶（MDH）。此外，RES上调了猪AdipoQ信号通路中的血浆AdipoQ浓度，AdipoQ，AdipoR1受体，AdipoR2，AMPK和PGC-1α的表达。此外，RES处理猪骨骼肌卫星细胞的结果表明，RES增加了AdipoR1，AMPK，PGC-1α，MyHC1和MyHC2a的表达，同时降低了MyHC2b的表达。尽管将RES添加到了细胞中，敲低AdipoR1表达不能增加AMPK和PGC-1α的表达。这项研究表明，RES通过AdipoR1-AMPK-PGC-1α途径增加了猪中氧化性肌纤维的比例。