A new cell line derived from dorsal fin of rabbit fish Siganus fuscescens was developed and characterized. The cell line was isolated from the dorsal fin, named as rabbit fish fin (RFF) cell line, and which was sub-cultured for 50 cycles since the development. This cell line was tested for growth in different temperatures and serum concentrations, and the best growing condition was at 20% serum at 28 °C. In cultured RFF cells, amplification of 18S rRNA from genomic DNA and immunostaining of cellular cytokeratin confirmed the proper identity of S. fuscescens fish. After 30th passage of cultures, the cells were exposed to challenge of inflammation, triggered by LPS, and hypoxia, mimicked by CoCl₂. Cultured RFF cells showed robust sensitive responses to inflammation and hypoxia in directing the expressions of cytokines and hypoxia inducible factor-1α (HIF-1α). The water extract of aerial part of Scutellaria baicalensis (SBA) has been shown in rabbit fish to prevent inflammation. Here, we extended this notion of testing the efficacy of SBA extract in the developed cultured RFF cells. Application of SBA extract inhibited the expression of LPS-induced inflammatory cytokines, i.e. IL-1β, IL-6, as well as the signaling of NF-κB. The application of CoCl₂ in cultured RFF cells triggered the hypoxia-induced cell death and up regulation of HIF-1α. As expected, applied SBA extract in the cultures prevented the hypoxia-induced signaling. Our results show the established RFF cell line may be served as an ideal in vitro model in drug screening relating to inflammation and hypoxia. Additionally, we are supporting the usage of SBA herbal extract in fish aquaculture, which possesses efficacy against inflammation and hypoxia.

开发并鉴定了一种源自兔鱼Siganus fuscescens背鳍的新细胞系。该细胞系从背鳍中分离得到，命名为兔鱼鳍（RFF）细胞系，自发育后传代培养50个循环。该细胞系在不同温度和血清浓度下进行生长测试，最佳生长条件是28℃、20%血清。在培养的 RFF 细胞中，基因组 DNA 中的 18S rRNA 扩增和细胞角蛋白的免疫染色证实了S. fuscescens鱼的正确身份。培养物传代第 30 代后，细胞暴露于由 LPS 引发的炎症和由 CoCl ₂模拟的缺氧条件下。培养的 RFF 细胞在指导细胞因子和缺氧诱导因子 1α (HIF-1α) 的表达方面对炎症和缺氧表现出强烈的敏感反应。黄芩地上部分的水提取物（SBA）已被证明可以在兔鱼中预防炎症。在这里，我们扩展了测试 SBA 提取物在开发的培养 RFF 细胞中的功效的概念。 SBA 提取物的应用抑制了 LPS 诱导的炎症细胞因子（即 IL-1β、IL-6）的表达以及 NF-κB 信号传导。 CoCl ₂在培养的RFF细胞中的应用引发了缺氧诱导的细胞死亡和HIF-1α的上调。正如预期的那样，在培养物中应用 SBA 提取物可以阻止缺氧诱导的信号传导。我们的结果表明，建立的 RFF 细胞系可以作为炎症和缺氧相关药物筛选的理想体外模型。 此外，我们支持在鱼类养殖中使用SBA草药提取物，它具有抗炎症和缺氧的功效。