Tefluthrin was the first pyrethroid developed for soil treatment. There was no report about the toxicity to terrestrial invertebrates at the enantiomer level. The main objective of the present study was to investigate the enantiomer-specific acute toxicity to the earthworm Eisenia fetida and potential mechanism via multilevel response. The filter paper contact and the artificial soil method were used to detect the acute toxicity of tefluthrin enantiomers to earthworms. Histopathological examination (H&E), biochemical criterion, and comet assay were used to identify the effects and potential mechanism of toxicity. The order of acute toxicity was Z-cis-(1S,3S)-(−)-tefluthrin < Rac-tefluthrin < Z-cis-(1R,3R)-(+)-tefluthrin. H&E stained images showed that intestinal cells were suffered seriously damaged after exposed to Rac-tefluthrin, and the Z-cis-(1R,3R)-(+)-isomer. Tefluthrin and enantiomers also enantioselectively disturbed reactive oxygen species (ROS) level and enzymatic activity. Additionally, Z-cis-(1R,3R)-(+)-tefluthrin significantly increased the olive tail moment (OTM) and Trail DNA% compared with the control and other treatment groups at the concentration of 0.1 mg/kg was observed. It can be concluded that intestinal damage, body weight changes, DNA damage caused by oxidative stress that might be the primary mechanisms of tefluthrin toxicity to earthworms. The results indicated the rational use of chiral compounds in agriculture to avoid damage to the soil ecosystem.

Tefluthrin是第一种用于土壤处理的拟除虫菊酯。没有关于对映体水平对陆生无脊椎动物毒性的报道。本研究的主要目的是通过多级反应研究对映体对f艾美丝虫的急性毒性和潜在机制。采用滤纸接触法和人工土壤法检测了替氟菊酯对映体对earth的急性毒性。使用组织病理学检查（H＆E），生化指标和彗星试验来确定毒性的影响和潜在机理。急性毒性的顺序为Z-顺式- （1-小号，3小号） - （ - ） -七氟菊酯<  Rac的-tefluthrin <Z-顺式-（1 R，3 R）-（+）-氟乐灵。H＆E染色图像显示，暴露于Rac -tefluthrin和Z-顺式-（1 R，3 R）-（+）-异构体后，肠道细胞受到严重破坏。Tefluthrin和对映异构体还对映选择性干扰活性氧（ROS）水平和酶活性。另外，Z-顺式-（1 R，3 R与对照组和其他处理组相比，在0.1 mg / kg的浓度下，）-（+）-特氟菊酯显着增加了橄榄尾矩（OTM）和Trail DNA％。可以得出结论，氧化应激引起的肠道损伤，体重变化，DNA损伤可能是替氟洛林对earth毒性的主要机制。结果表明，在农业中合理使用手性化合物可避免破坏土壤生态系统。