The aim of this study was the separation of cholic, ursodeoxycholic, chenodeoxycholic, deoxycholic, and lithocholic bile acids by thin-layer chromatography (TLC) with mobile phases based on surfactants. The effect of surfactant type and concentration, the pH of the mobile phase, the type and amount of the mobile phase modifier, and the effect of temperature and type of material backed silica gel TLC layers on the investigated bile acid separation were studied. The separation was carried out on normal-phase TLC plates Sorbfil PTLC-P-A-UV-254 and Sorbfil PTLC-AF-A-UV-254. The acid zones after separation were visualized by using sulfuric acid solutions followed by heating the TLC plate and acid spots observing under 365-nm ultraviolet (UV) light. Mobile phases based on surfactants, namely, cationic cetylpyridinium chloride, anionic sodium dodecyl sulfate, and nonionic Tween-80, were used. Aliphatic alcohols 1-propanol, 1-butanol, and 1-pentanol as well as the nonionic surfactant Tween-80 were used as modifiers of the mobile phase. The optimal separation of the studied acids was achieved using the two-dimensional TLC method with sequential elution with mobile phases based on cetylpyridinium chloride, first with the addition of 1-butanol and then, perpendicular to the initial direction, with the addition of 1-pentanol. The proposed method for the bile acid separation can be used in the analysis of human biological fluids and drugs.

中文翻译：

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基于表面活性剂的流动相薄壁色谱分离多种胆汁酸

这项研究的目的是通过基于表面活性剂的流动相薄层色谱法（TLC）分离胆酸，熊去氧胆酸，鹅去氧胆酸，脱氧胆酸和石脑胆汁酸。研究了表面活性剂类型和浓度，流动相的pH值，流动相改性剂的类型和量以及背衬硅胶TLC层的温度和类型对所研究的胆汁酸分离的影响。分离在正相薄层色谱板Sorbfil PTLC-PA-UV-254和Sorbfil PTLC-AF-A-UV-254上进行。通过使用硫酸溶液，然后加热TLC板和在365 nm紫外（UV）光下观察到的酸点，可以观察到分离后的酸区。基于表面活性剂的流动相，即阳离子十六烷基吡啶鎓氯化物，使用阴离子十二烷基硫酸钠和非离子Tween-80。脂族醇1-丙醇，1-丁醇和1-戊醇以及非离子表面活性剂Tween-80用作流动相的改性剂。研究的酸的最佳分离是通过二维TLC方法实现的，依次使用基于鲸蜡基吡啶鎓氯化物的流动相进行洗脱，首先添加1-丁醇，然后垂直于初始方向添加1-戊醇。所提出的胆汁酸分离方法可用于分析人体生物流体和药物。研究的酸的最佳分离是通过二维TLC方法实现的，依次使用基于鲸蜡基吡啶鎓氯化物的流动相进行洗脱，首先添加1-丁醇，然后垂直于初始方向添加1-戊醇。所提出的胆汁酸分离方法可用于分析人体生物流体和药物。研究的酸的最佳分离是通过二维TLC方法实现的，依次使用基于鲸蜡基吡啶鎓氯化物的流动相进行洗脱，首先添加1-丁醇，然后垂直于初始方向添加1-戊醇。所提出的胆汁酸分离方法可用于分析人体生物流体和药物。