An immobilized metal affinity (IMA) adsorbent was prepared by grafting bottlebrush polymer pendant with iminodiacetic acid (IDA) from the surface of polydopamine (PDA)-coated magnetic graphene oxide (magGO), via surface-initiated atom transfer radical polymerization (SI-ATRP). Poly(hydroxyethyl methacrylate) (PHEMA) was grafted firstly from the PDA-coated magGO as the backbone, and then poly(glycidyl methacrylate) was grafted from the PHEMA chains via the second SI-ATRP to afford the bottlebrush polymer–grafted magGO Thereafter, IDA was anchored on the nanocomposites to produce the IMA adsorbent after chelating copper ions. The adsorbent was characterized by various physical and physicochemical methods. Its adsorption properties were evaluated by using histidine-rich proteins (bovine hemoglobin, BHb) and other proteins (lysozyme and cytochrome-C). The results show that its maximum adsorption capacity to BHb was 378.6 mg g−1, and the adsorption equilibrium can be quickly reached within 1 h. The adsorbent has excellent reproducibility and reusability. It has been applied to selectively purify hemoglobin from human whole blood, indicating its potential in practical applications. Graphical abstract Graphical abstract

中文翻译：

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瓶刷聚合物改性磁性石墨烯作为固定金属离子亲和吸附剂的制备，用于纯化血样中的血红蛋白

通过表面引发原子转移自由基聚合（SI-ATRP）从聚多巴胺（PDA）包覆的磁性氧化石墨烯（magGO）表面接枝瓶刷聚合物悬垂物与亚氨基二乙酸（IDA）制备固定化金属亲和（IMA）吸附剂）。聚（甲基丙烯酸羟乙酯）（PHEMA）首先从 PDA 包覆的 magGO 作为骨架接枝，然后通过第二个 SI-ATRP 从 PHEMA 链上接枝聚（甲基丙烯酸缩水甘油酯），得到瓶刷聚合物接枝的 magGO。在螯合铜离子后，IDA 被锚定在纳米复合材料上以产生 IMA 吸附剂。吸附剂通过各种物理和物理化学方法进行表征。通过使用富含组氨酸的蛋白质（牛血红蛋白，BHb）和其他蛋白质（溶菌酶和细胞色素-C）评估其吸附性能。结果表明，其对BHb的最大吸附量为378.6 mg g-1，1 h内即可快速达到吸附平衡。该吸附剂具有优良的重现性和重复使用性。它已被应用于从人类全血中选择性纯化血红蛋白，表明其在实际应用中的潜力。图形摘要图形摘要