A most important parameter in tissue engineering of blood vessels is the restoration of the endothelium in small-diameter vascular grafts. Not only the mechanical properties of the inner layer of the synthetic graft but also its physical and chemical properties play important roles in both the response of the endothelial cells and the successful reconstruction of the lumen layer. This study was conducted to develop an optimized scaffold of pre-polyglycerol sebacate-poly lactic acid (pre-PGS-PLLA) polymeric blend and to determine the relevant electrospinning parameters that guarantee the required tensile strength, failure strain, and Young’s modulus of the graft. A second aspect of the study involved evaluation of the suture strength, burst strength, water contact angle, degradation rate, and the response of Human Umbilical Vein Endothelial Cells to the fabricated scaffold. The pre-PGS-PLLA blend showed a tensile strength of 0.420 ± 0.23 MPa, a Young’s modulus of 2.05 ± 0.27 MPa, a suture strength with a failure strain of 67.6 ± 10.97%, and a burst strength of 90.2 ± 0.95 kPa, all of which were mechanically consistent with the artery intima characteristics. Moreover, a contact angle of > 10° with water was recorded. The scaffold was found capable of maintaining 40.14% of its initial weight after 60 days while also retaining its structural integrity. Based on the HUVEC cell response examination, the scaffold had no toxicity and the cells showed satisfactory morphology and proliferation. Given the proper imitation of the mechanical properties as well as the survival and proliferation of the endothelial cells, the synthesized scaffold may be recommended for the reconstruction of coronary artery intima and the treatment of vascular tissue disorders.

血管组织工程中最重要的参数是小直径血管移植物中内皮的恢复。不仅人造移植物内层的机械性能，而且其物理和化学性能在内皮细胞的反应和内腔层的成功重建中都起着重要作用。进行这项研究的目的是开发癸二酸甘油三酯-聚乳酸（pre-PGS-PLLA）聚合物共混物的优化支架，并确定相关的静电纺丝参数，以确保所需的抗张强度，破坏应变和接枝杨氏模量。研究的第二个方面涉及评估缝合线强度，破裂强度，水接触角，降解率，和人脐静脉内皮细胞对预制支架的反应。PGS-PLLA之前的混合物的抗张强度为0.420±0.23 MPa，杨氏模量为2.05±0.27 MPa，缝合线强度为67.6±10.97％，断裂强度为90.2±0.95 kPa其中在机械上与动脉内膜特征一致。此外，记录到与水的接触角＞ 10°。发现该支架能够在60天后保持其初始重量的40.14％，同时还保持其结构完整性。根据HUVEC细胞反应检查，该支架没有毒​​性，并且细胞表现出令人满意的形态和增殖。如果适当模仿机械特性以及内皮细胞的存活和增殖，