The recent advancement in genome sequencing facilities, proteomics, transcriptomics, and metabolomics of eukaryotes have opened door for employment of molecular diagnostic techniques for early detection of parasites and determining target molecules for formulating control strategies. It further leads to the introduction of several purified vaccines in the field of veterinary parasitology. Earlier, the conventional diagnostic methods was entirely based upon morphological taxonomy for diagnosis of parasites but nowadays improved molecular techniques help in phylogenetic study and open an another area of molecular taxonomy of parasites with high precision. Control measures based upon targeting endosymbionts in parasites like Dirofilaria immitis is also under exploration in veterinary parasitology. Metagenomics have added an inside story of parasites bionomics which have created havoc in human and animals population since centuries. Omics era is playing a key role in opening the new approaches on parasite biology. Various newer generations of safer vaccines like edible vaccines and subunit vaccines and diagnostic techniques based upon purified immunologically active epitopes have become commercially available against the parasites (helminths, protozoa and arthropod borne diseases). Nowadays, a transgenic and gene knock out studies using RNA interference and CRISPR are also helping in understanding the functions of genes and screening of target genes, which are not available before the advent of molecular tools. Molecular techniques had paramount impact on increasing the sensitivity of diagnostic tools, epidemiological studies and more importantly in controlling these diseases. This review is about the advancements in veterinary parasitology and their impact on the control of these pathogens.

中文翻译：

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分子工具——控制具有兽医重要性的寄生虫的进展、机会和前景

基因组测序设备、蛋白质组学、转录组学和真核生物代谢组学的最新进展为使用分子诊断技术早期检测寄生虫和确定用于制定控制策略的目标分子打开了大门。它进一步导致在兽医寄生虫学领域引入了几种纯化疫苗。早期，传统的诊断方法完全基于形态分类学来诊断寄生虫，但现在改进的分子技术有助于系统发育研究，开辟了另一个高精度的寄生虫分子分类学领域。在兽医寄生虫学中也正在探索基于针对寄生虫（如犬恶丝虫）中的内共生菌的控制措施。宏基因组学增加了寄生虫生物组学的内幕故事，几个世纪以来一直在人类和动物种群中造成严重破坏。组学时代在开启寄生虫生物学新方法方面发挥着关键作用。各种新一代更安全的疫苗，如可食用疫苗和亚单位疫苗以及基于纯化的免疫活性表位的诊断技术，已经在商业上可用于对抗寄生虫（蠕虫、原生动物和节肢动物传播的疾病）。如今，利用 RNA 干扰和 CRISPR 进行的转基因和基因敲除研究也有助于了解基因的功能和筛选靶基因，这在分子工具出现之前是不可用的。分子技术对提高诊断工具的敏感性有着至关重要的影响，流行病学研究，更重要的是控制这些疾病。这篇综述是关于兽医寄生虫学的进展及其对控制这些病原体的影响。