Bacteria belonging to the family Anaplasmataceae cause infections in humans and domestic animals. The consequences of infection can be significant economic losses for farmers. To better understand the epidemiology of tick-borne Anaplasmataceae in Corsica, we used molecular methods to detect and characterize Anaplasmataceae in ixodid ticks collected from cattle. Anaplasmataceae were detected by using a real-time polymerase chain reaction (PCR) targeting the 23S rRNA gene. Partial sequencing of rpoB and groEL allowed identifying species and conducting phylogenetic analyses. Infection rates were calculated using maximum likelihood estimation (MLE) with 95% confidence intervals (CIs). In total, 597 Rhipicephalus bursa, 216 Hyalomma marginatum, and seven Ixodes ricinus were collected from cattle during July–August 2017 and July–December 2018. Overall, Anaplasmataceae DNA was detected in 15 of 255 tick pools (MLE = 1.7%; 95% CI 0.9–2.7%). The molecular analysis revealed two species within the genus Anaplasma: A. marginale and A. phagocytophilum. We also detected bacteria within the genus Ehrlichia: we confirmed the detection of E. minasensis DNA in H. marginatum and R. bursa tick pools collected from cattle in Corsica and detected, for the first time to our knowledge, Candidatus E. urmitei in Corsican R. bursa ticks and a potential new species, Candidatus E. corsicanum. Further studies are needed to ascertain the pathogenesis and zoonotic potential of the strains and their importance for animals and public health.

中文翻译：

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在法国科西嘉岛的牛身上采集的s中的无核科的分子筛查。

属于Anaplasmataceae科的细菌在人类和家畜中引起感染。感染的后果可能给农民造成巨大的经济损失。为了更好地了解科西嘉of传播的无形纲科的流行病学，我们使用了分子方法来检测和鉴定从牛身上收集的ix类tick科的无形纲科。通过使用针对23S rRNA基因的实时聚合酶链反应（PCR）检测无形科。rpoB和g roEL的部分测序可以鉴定物种并进行系统发育分析。使用最大似然估计（MLE）和95％置信区间（CIs）计算感染率。总共597头颅蛇头，216透明膜缘，七个篦子硬蜱从牛七八月2017年七月至十二月2018总体期间收集的，无形体DNA在255的15蜱池（95％CI 0.9-2.7％MLE = 1.7％）中检测到。分子分析表明属内两种细胞无形体：A.边缘无和A.吞噬细胞。我们还发现属内的细菌细胞埃立克体：我们证实了检测E. minasensis DNA在H. marginatum和R.囊蜱在科西嘉岛牛收集池和检测，首次就我们所知，暂定E. urmitei在科西嘉伯萨河蜱和潜在的新种，暂定E. corsicanum。需要进一步研究以确定该菌株的发病机理和人畜共患病潜力及其对动物和公共卫生的重要性。