Abstract

Nitric-oxide synthase activity was detected in Neurospora crassa cells with the measurement of the conversion of ³H-L-arginine to ³H-L-citrulline. Some characteristics of this activity (sensitivity to calcium ions, action of specific enzyme inhibitors, and western blot analysis) were similar to those of an inducible enzyme found in mammals. According to western blotting, the molecular weight of NO synthase was around 130 kDa. No light-dependent changes in the specific activity of NO synthase were revealed in the photocarotenogenesis and photoconidiation of N. сrassa.

中文翻译：

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一氧化氮合酶活性在神经孢子囊的光形态发生中

摘要

通过测量³ H-L-精氨酸向³ H-L-瓜氨酸的转化，在神经孢霉细胞中检测到一氧化氮合酶活性。这种活性的某些特征（对钙离子的敏感性，特定酶抑制剂的作用以及蛋白质印迹分析）与哺乳动物中发现的可诱导酶相似。根据蛋白质印迹法，NO合酶的分子量约为130kDa。在N.сrassa的光致血管生成和光化过程中，没有发现NO合酶比活性的光依赖性变化。