Abstract

The possible role of hydrogen sulfide as a signal mediator in the induction of heat resistance of wheat (Triticum aestivum L.) plantlets by salicylic acid (SA) has been studied. The treatment of plantlets with SA (1 and 10 μM) or a hydrogen sulfide donor (NaHS, 0.1 and 0.25 mM) increased their resistance to damaging heating (10 min at 45°C). Under the influence of SA, a transient increase in the hydrogen sulfide content occurred in roots with the maximum effect in 2–3 h after the start of treatment. The treatment of roots with SA increased the activity of superoxide dismutase (SOD), catalase, and guaiacol peroxidase in them. Under the influence of the hydrogen sulfide donor NaHS, the activity of SOD and catalase increased significantly. Also, the treatment of roots with SA and NaHS reduced the effect of the accumulation of the product of lipid peroxidation, malondialdehyde, which is caused by heating. The inhibitors of hydrogen sulfide synthesis, hydroxylamine and potassium pyruvate, partially eliminated the effects of the increase in the activity of antioxidant enzymes and the development of heat resistance caused by SA. At the same time, the combined treatment of intact plantlets with 10 μM SA and 0.1 mM NaHS contributed to the additional increase in the activity of antioxidant enzymes and increased plantlet survival after heating. It is concluded that hydrogen sulfide participates in the implementation of the protective influence of SA on wheat plantlets under heat stress.

中文翻译：

---

硫化氢参与小麦幼苗根系抗氧化系统的诱导及其水杨酸的耐热性

摘要

硫化氢的可能作用如在小麦的耐热性的感应的信号介体（小麦已经研究了通过水杨酸（SA）培养的L.）幼苗。用SA（1和10μM）或硫化氢供体（NaHS，0.1和0.25 mM）处理小植株增加了其对破坏性加热的抵抗力（在45°C下10分钟）。在SA的影响下，根部的硫化氢含量瞬时增加，开始处理后2-3小时内效果最大。用SA处理根部可以提高其中的超氧化物歧化酶（SOD），过氧化氢酶和愈创木酚过氧化物酶的活性。在硫化氢供体NaHS的影响下，SOD和过氧化氢酶的活性显着增加。同样，用SA和NaHS处理根部降低了由加热引起的脂质过氧化产物丙二醛积聚的影响。硫化氢合成的抑制剂，羟胺和丙酮酸钾部分消除了由SA引起的抗氧化酶活性增加和耐热性发展的影响。同时，完整植株与10μMSA和0.1 mM NaHS的联合处理有助于进一步提高抗氧化酶的活性，并增加加热后植株的存活率。结论是，硫化氢参与了SA对热胁迫下小麦幼苗的保护作用。1 mM NaHS有助于进一步提高抗氧化酶的活性，并增加加热后的幼苗存活率。结论是，硫化氢参与了SA对热胁迫下小麦幼苗的保护作用。1 mM NaHS有助于进一步提高抗氧化酶的活性，并增加加热后的幼苗存活率。结论是：硫化氢参与了SA对热胁迫下小麦幼苗的保护作用。