The simultaneous determination of ascorbic acid, rutin and quercetin in the food and health products on the glassy carbon electrode have been investigated by adsorptive stripping differential pulse voltammetry (AdSDPV) with the aid of chemometrics. In the pH 5.2 KH₂PO₄ – Na₂HPO₄ buffer solution, both rutin and quercetin has shown a pair of sensitive reversible oxidation-reduction peak, while ascorbic acid only has shown an irreversible oxidation peak on the glassy carbon electrode. In the range of 0.35-0.50 V, the vlotammograms of three components has shown serious overlap with peak potential of ascorbic acid, rutin and quercetin. So, it is extremely difficult to realize direct measurement for the content of single component. Chemometrics methods have been introduced to determine the admixture of the three components. In this way, we have avoided the troublesome procedures of separation and purification, and assay the artificial compound of the three components all at once. We have estimated the three components in the food and the health products with satisfactory results.

中文翻译：

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吸附溶出差分脉冲伏安法与化学计量学在食品和保健品中抗坏血酸，芦丁和槲皮素的测定中的应用

借助于化学计量学，通过吸附溶出差分脉冲伏安法（AdSDPV）研究了同时测定玻璃碳电极上食品和保健品中抗坏血酸，芦丁和槲皮素的方法。在pH 5.2 KH ₂ PO ₄ – Na ₂ HPO _4中在缓冲溶液中，芦丁和槲皮素均显示出一对敏感的可逆氧化还原峰，而抗坏血酸仅在玻璃碳电极上显示出不可逆氧化峰。在0.35-0.50 V的范围内，三种成分的伏安图均显示出与抗坏血酸，芦丁和槲皮素的峰值电势严重重叠。因此，要实现对单组分含量的直接测量是极其困难的。已经引入化学计量学方法来确定这三种组分的混合物。这样，我们避免了麻烦的分离和纯化步骤，并且可以一次测定所有三种成分的人工化合物。我们估计了食品和保健品中的三个成分，结果令人满意。