Background The advent and boom of DNA barcoding technologies have provided a powerful tool for the fields of ecology and systematics. Here, we present the InBIO Barcoding Initiative Database: Portuguese Bats (Chiroptera) dataset containing DNA sequences of 63 specimens representing the 25 bat species currently known for continental Portugal. For that, we sequenced tissues samples obtained in a vast array of projects spanning the last two decades. New information We added four new Barcoding Index Numbers (BINs) to existing Chiroptera barcodes on BOLD, two belonging to Myotis escalerai, one to Plecotus auritus and the other to Rhinolophus hipposideros. Surprisingly, one of the samples initially identified in the field as Myotis mystacinus turned out to be Myotis alcathoe, which represents the first record of this species for Portugal. The presence of Nyctalus noctula in Portugal was also genetically confirmed for the first time. This case study shows the power and value of DNA barcoding initiatives to unravel new data that may be hidden on biological collections.

中文翻译：

---

藏在我们的口袋里：建立一个 DNA 条形码库为葡萄牙揭开了第一个 Myotis alcathoe 的记录。

背景 DNA 条形码技术的出现和繁荣为生态学和系统学领域提供了强有力的工具。在这里，我们展示了 InBIO 条形码倡议数据库：葡萄牙蝙蝠 (Chiroptera) 数据集，其中包含 63 个样本的 DNA 序列，这些样本代表目前葡萄牙大陆已知的 25 种蝙蝠物种。为此，我们对过去二十年的大量项目中获得的组织样本进行了测序。新信息 我们在 BOLD 上的现有 Chiroptera 条形码中添加了四个新的条形码索引号 (BIN)，两个属于 Myotis escalerai，一个属于 Plecotus auritus，另一个属于 Rhinolophus hipposideros。令人惊讶的是，最初在该领域鉴定为 Myotis mystacinus 的样本之一竟然是 Myotis alcathoe，这代表了葡萄牙该物种的第一个记录。Nyctalus noctula 在葡萄牙的存在也首次在基因上得到证实。本案例研究展示了 DNA 条形码计划在解开可能隐藏在生物收藏品中的新数据方面的力量和价值。