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Insight into Molecular Epidemiology, Antimicrobial Resistance, and Virulence Genes of Extensively Drug-Resistant Acinetobacter baumannii in Thailand
Microbial Drug Resistance ( IF 2.6 ) Pub Date : 2021-03-12 , DOI: 10.1089/mdr.2020.0064 Phattaraporn Kongthai 1 , Rapee Thummeepak 1 , Udomluk Leungtongkam 1 , Renuka Pooarlai 1 , Thawatchai Kitti 2 , Aunchalee Thanwisai 1 , Narisara Chantratita 3 , Andrew D Millard 4 , Sutthirat Sitthisak 1, 5
Microbial Drug Resistance ( IF 2.6 ) Pub Date : 2021-03-12 , DOI: 10.1089/mdr.2020.0064 Phattaraporn Kongthai 1 , Rapee Thummeepak 1 , Udomluk Leungtongkam 1 , Renuka Pooarlai 1 , Thawatchai Kitti 2 , Aunchalee Thanwisai 1 , Narisara Chantratita 3 , Andrew D Millard 4 , Sutthirat Sitthisak 1, 5
Affiliation
Extensively drug-resistant Acinetobacter baumannii (XDR-AB) is a major threat to public health worldwide. A retrospective study for 27 XDR-AB isolates from four tertiary hospitals in Thailand was conducted. Beta-lactamase and virulence genes were characterized by PCR. The blaADC, blaOXA-51, and blaOXA-23 were detected in all isolates, whereas blaPER-1 and blaNDM-1 genes were present in 7.4% and 3.7% of isolates. All isolates had virulence genes, including genes in iron acquisition system, biofilm formation and secretion systems. The plasmids in XDR-AB belonged to GR2 (100%), GR6 (40.7%), and GR1 (7.4%). Multilocus sequence typing sequence types (STs) were further investigated. The data demonstrated that XDR-AB isolates had nine STs: ST195 (n = 4), ST208 (n = 4), ST368 (n = 1), ST451 (n = 5), ST457 (n = 2), ST1947 (n = 1), ST1166 (n = 7), including two novel STs namely ST1682 (n = 2) and ST1684 (n = 1). We observed that the majority ST1166 (25.9%) was associated with the prevalence of GR2 and GR6 plasmids and traU virulence gene. Genome-based single nucleotide polymorphism phylogenetic analysis of the isolates with two novel ST types indicated that the two isolates belonged to the international clone II (IC2) within the same cluster. In conclusion, our data showed the dissemination of XDR-AB isolates harbored virulence genes and antibiotic resistance genes among four hospitals in Thailand. The results highlighted the difficulty posed for the empirical treatment of the patients with the A. baumannii infection.
中文翻译：
深入了解泰国广泛耐药鲍曼不动杆菌的分子流行病学、抗菌素耐药性和毒力基因
广泛耐药鲍曼不动杆菌(XDR-AB) 是全球公共卫生的主要威胁。对来自泰国四家三级医院的 27 种 XDR-AB 分离株进行了回顾性研究。β-内酰胺酶和毒力基因通过PCR表征。的BLA ADC，BLA OXA-51，和BLA OXA-23在所有的分离物检测到，而BLA PER-1和BLA NDM-1基因存在于 7.4% 和 3.7% 的分离株中。所有分离株均具有毒力基因，包括铁获取系统、生物膜形成和分泌系统中的基因。XDR-AB 中的质粒属于 GR2 (100%)、GR6 (40.7%) 和 GR1 (7.4%)。进一步研究了多位点序列分型序列类型 (ST)。数据表明 XDR-AB 分离株具有 9 个 ST：ST195 ( n = 4)、ST208 ( n = 4)、ST368 ( n = 1)、ST451 ( n = 5)、ST457 ( n = 2)、ST1947 ( n = 1)、ST1166 ( n = 7)，包括两个新的 ST，即 ST1682 ( n = 2) 和 ST1684 ( n = 1)。我们观察到，大多数ST1166（25.9％）与GR2和GR6质粒的患病率和相关联的TR一个Ú毒力基因。基于基因组的单核苷酸多态性系统发育分析表明，这两个分离株属于同一簇内的国际克隆 II (IC2)。总之，我们的数据显示 XDR-AB 分离株在泰国四家医院中的传播带有毒力基因和抗生素抗性基因。结果强调了对鲍曼不动杆菌感染患者进行经验治疗带来的困难。
更新日期：2021-03-17
中文翻译：
深入了解泰国广泛耐药鲍曼不动杆菌的分子流行病学、抗菌素耐药性和毒力基因
广泛耐药鲍曼不动杆菌(XDR-AB) 是全球公共卫生的主要威胁。对来自泰国四家三级医院的 27 种 XDR-AB 分离株进行了回顾性研究。β-内酰胺酶和毒力基因通过PCR表征。的BLA ADC，BLA OXA-51，和BLA OXA-23在所有的分离物检测到，而BLA PER-1和BLA NDM-1基因存在于 7.4% 和 3.7% 的分离株中。所有分离株均具有毒力基因，包括铁获取系统、生物膜形成和分泌系统中的基因。XDR-AB 中的质粒属于 GR2 (100%)、GR6 (40.7%) 和 GR1 (7.4%)。进一步研究了多位点序列分型序列类型 (ST)。数据表明 XDR-AB 分离株具有 9 个 ST：ST195 ( n = 4)、ST208 ( n = 4)、ST368 ( n = 1)、ST451 ( n = 5)、ST457 ( n = 2)、ST1947 ( n = 1)、ST1166 ( n = 7)，包括两个新的 ST，即 ST1682 ( n = 2) 和 ST1684 ( n = 1)。我们观察到，大多数ST1166（25.9％）与GR2和GR6质粒的患病率和相关联的TR一个Ú毒力基因。基于基因组的单核苷酸多态性系统发育分析表明，这两个分离株属于同一簇内的国际克隆 II (IC2)。总之，我们的数据显示 XDR-AB 分离株在泰国四家医院中的传播带有毒力基因和抗生素抗性基因。结果强调了对鲍曼不动杆菌感染患者进行经验治疗带来的困难。
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