Interactions between DNA and the Hfq Amyloid-like Region Trigger a Viscoelastic Response.,Biomacromolecules

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Interactions between DNA and the Hfq Amyloid-like Region Trigger a Viscoelastic Response.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-07-28 , DOI: 10.1021/acs.biomac.0c00747
Omar El Hamoui ₁ , Indresh Yadav ₂ , Milad Radiom ₃ , Frank Wien ₁ , Jean-Francois Berret ₃ , Johan R C van der Maarel ₂ , Véronique Arluison _{4,

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Affiliation

Molecular transport of biomolecules plays a pivotal role in the machinery of life. Yet, this role is poorly understood due the lack of quantitative information. Here, the role and properties of the C-terminal region of Escherichia coli Hfq is reported, involved in controlling the flow of a DNA solution. A combination of experimental methodologies has been used to probe the interaction of Hfq with DNA and to measure the rheological properties of the complex. A physical gel with a temperature reversible elasticity modulus is formed due to the formation of noncovalent cross-links. The mechanical response of the complexes shows that they are inhomogeneous soft solids. Our experiments indicate that the Hfq C-terminal region could contribute to the genome’s mechanical response. The reported viscoelasticity of the DNA-protein complex might have implications for cellular processes involving molecular transport of DNA or segments thereof.

中文翻译：

DNA与Hfq淀粉样蛋白区域之间的相互作用触发了粘弹性反应。

生物分子的分子运输在生命机制中起着关键作用。然而，由于缺乏定量信息，人们对该角色的了解甚少。在此，大肠杆菌C末端区域的作用和特性据报道，Hfq参与控制DNA溶液的流动。实验方法的组合已用于探测Hfq与DNA的相互作用并测量复合物的流变特性。由于非共价交联的形成，形成了具有温度可逆弹性模量的物理凝胶。配合物的机械反应表明它们是不均匀的软固体。我们的实验表明，Hfq C末端区域可能有助于基因组的机械反应。报道的DNA-蛋白质复合物的粘弹性可能对涉及DNA或其片段的分子运输的细胞过程有影响。

更新日期：2020-09-14

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