Sequential Self-Assembly Using Tannic Acid and Phenylboronic Acid-Modified Copolymers for Potential Protein Delivery.,Biomacromolecules

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Sequential Self-Assembly Using Tannic Acid and Phenylboronic Acid-Modified Copolymers for Potential Protein Delivery.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-07-27 , DOI: 10.1021/acs.biomac.0c00903
Yuto Honda _{1,

2} , Takahiro Nomoto _{1,

2} , Makoto Matsui ₁ , Hiroyasu Takemoto _{1,

2} , Yuka Kaihara _{1,

2} , Yutaka Miura _{1,

2} , Nobuhiro Nishiyama _{1,

2,

3}

Affiliation

Tannic acid (TA) can form stable complexes with proteins, attracting significant attention as protein delivery systems. However, its systemic application has been limited due to nonspecific interaction. Here, we report a simple technique to prepare systemically applicable protein delivery systems using sequential self-assembly of a protein, TA, and phenylboronic acid-conjugated PEG-poly(amino acid) block copolymers in aqueous solution. Mixing the protein and TA in aqueous solution led to covering of the protein with TA, and subsequent addition of the copolymer resulted in the formation of boronate esters between TA and copolymers, constructing the core–shell-type ternary complex. The ternary complex covered with PEG exhibited a small hydrodynamic diameter of ∼10–20 nm and prevented an unfavorable interaction with serum components, thereby accomplishing significantly prolonged blood circulation and enhanced tumor accumulation in a subcutaneous tumor model. The technique utilizing supramolecular self-assembly may serve as a novel approach for designing protein delivery systems.

中文翻译：

使用鞣酸和苯硼酸改性的共聚物进行的顺序自组装，用于潜在的蛋白质传递。

单宁酸（TA）可以与蛋白质形成稳定的复合物，作为蛋白质递送系统备受关注。然而，由于非特异性相互作用，其系统应用受到限制。在这里，我们报告了一种简单的技术，可以使用水溶液中的蛋白质，TA和苯基硼酸共轭的PEG-聚（氨基酸）嵌段共聚物进行顺序自组装来制备系统适用的蛋白质递送系统。在水溶液中混合蛋白质和TA导致蛋白质被TA覆盖，随后加入共聚物导致TA和共聚物之间形成硼酸酯，从而构成核-壳型三元复合物。用PEG覆盖的三元复合物的流体动力学直径较小，约为10–20 nm，并且可以防止与血清成分的不利相互作用，从而在皮下肿瘤模型中显着延长了血液循环并增强了肿瘤的积累。利用超分子自组装的技术可以用作设计蛋白质递送系统的新方法。

更新日期：2020-09-14

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