Cholesteryl Ester Liquid Crystal Nanofibers for Tissue Engineering Applications,ACS Materials Letters

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Cholesteryl Ester Liquid Crystal Nanofibers for Tissue Engineering Applications
ACS Materials Letters ( IF 9.6 ) Pub Date : 2020-07-27 , DOI: 10.1021/acsmaterialslett.0c00224
Amir Nasajpour _{1,

2} , Azadeh Mostafavi ₃ , Adrian Chlanda ₄ , Chiara Rinoldi ₄ , Sina Sharifi ₅ , Matthew S. Ji _{1,

2} , Matthew Ye _{1,

2} , Steven J. Jonas _{1,

6} , Wojciech Swieszkowski ₄ , Paul S. Weiss _{1,

2,

7,

8} , Ali Khademhosseini _{1,

8,

9,

10,

11} , Ali Tamayol _{3,

12}

Affiliation

California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States
Department of Chemistry & Biochemistry, University of California, Los Angeles, Los Angeles, Los Angeles 90095, United States
Department of Mechanical and Materials Engineering, University of Nebraska, Lincoln, Nebraska 68588, United States
Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, 141 Woloska Str., 02-507 Warsaw, Poland
Schepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, United States
Department of Pediatrics, Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, Children’s Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, California 90095, United States
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States
Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, United States
Department of Radiology, University of California, Los Angeles, Los Angeles, California 90095, United States
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States
Terasaki Institute for Biomedical Innovation, Los Angeles, California 90049, United States
Department of Biomedical Engineering, University of Connecticut, Farmington, Connecticut 06030, United States

Liquid-crystal-based biomaterials provide promising platforms for the development of dynamic and responsive interfaces for tissue engineering. Cholesteryl ester liquid crystals (CLCs) are particularly well suited for these applications, due to their roles in cellular homeostasis and their intrinsic ability to organize into supramolecular helicoidal structures on the mesoscale. Here, we developed a nonwoven CLC electrospun scaffold by dispersing three cholesteryl ester-based mesogens within polycaprolactone (PCL). We tuned the ratio of our mesogens so that the CLC would be in the mesophase at the cell culture incubator temperature of 37 °C. In these scaffolds, the PCL polymer provided an elastic bulk matrix while the homogeneously dispersed CLC established a viscoelastic fluidlike interface. Atomic force microscopy revealed that the 50% (w/v) cholesteryl ester liquid crystal scaffold (CLC-S) exhibited a mesophase with topographic striations typical of liquid crystals. Additionally, the CLC-S favorable wettability and ultrasoft fiber mechanics enhanced cellular attachment and proliferation. Increasing the CLC concentration within the composites enhanced myoblast adhesion strength promoted myofibril formation in vitro with mouse myoblast cell lines.

中文翻译：

用于组织工程应用的胆固醇酯液晶纳米纤维

液晶生物材料为组织工程的动态和响应界面的开发提供了有希望的平台。胆固醇酯液晶（CLC）特别适合于这些应用，因为它们在细胞动态平衡中的作用以及它们在中尺度上组织为超分子螺旋结构的内在能力。在这里，我们通过在聚己内酯（PCL）中分散三种基于胆固醇酯的液晶元，开发了一种非织造CLC电纺支架。我们调整了液晶元的比例，以便在细胞培养箱温度为37°C时CLC处于中间相。在这些支架中，PCL聚合物提供了弹性体基质，而均匀分散的CLC建立了粘弹性的类流体界面。原子力显微镜显示50％（w / v）胆固醇酯液晶支架（CLC-S）表现出中间相，具有液晶典型的形貌条纹。此外，CLC-S良好的润湿性和超柔软的纤维力学增强了细胞的附着和增殖。复合材料中CLC浓度的增加增强了成肌细胞的粘附强度，促进了肌原纤维的形成小鼠成肌细胞体外培养。

更新日期：2020-09-08

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