ABSTRACT In this study, a high sensitivity and specificity monoclonal antibody (mAb) was developed based on the hydroxy and carboxy-termini of ochratoxin A (OTA). A fluorescent microsphere immunochromatographic test strip (FM-ICTS) was assembled for the rapid detection the OTA in rice flour. Some relevant parameters were optimized including the best concentration of coating antigen and optimal diluent buffer composition. Under optimal conditions, the strip assay had a visual limit of detection (LOD) of 2 ng/mL and a cut-off value of 16 ng/mL for OTA in rice flour samples. By means of a strip scan reader, we obtained a quantitative measurement for OTA with an LOD of 1.27 ng/mL. No significant difference in the strip test results was seen when compared to an ic-ELISA assay or LC–MS/MS. This also shows, therefore, that the FM-ICTS has potential application for rapid and accurate quantitative detection in the market place.

中文翻译：

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使用荧光微球免疫层析试纸条法快速灵敏地检测米粉中的赭曲霉毒素 A

摘要 在本研究中，基于赭曲霉毒素 A (OTA) 的羟基和羧基末端开发了一种高灵敏度和特异性的单克隆抗体 (mAb)。组装荧光微球免疫层析试纸（FM-ICTS）用于米粉中OTA的快速检测。优化了一些相关参数，包括最佳包被抗原浓度和最佳稀释缓冲液组成。在最佳条件下，条带测定法的视觉检测限 (LOD) 为 2 ng/mL，米粉样品中 OTA 的临界值为 16 ng/mL。通过条带扫描阅读器，我们获得了 OTA 的定量测量结果，LOD 为 1.27 ng/mL。与 ic-ELISA 测定或 LC-MS/MS 相比，条带测试结果没有显着差异。因此，这也表明，