ABSTRACT As a severely harmful zoonotic disease, toxoplasmosis has complex clinical symptoms with atypical lesions, thus making it difficult for diagnosis in a timely manner. Therefore, developing an appropriate technique to diagnose and prevent toxoplasmosis has become a subject of many studies. In this study, a colloidal gold immunochromatographic test strips for the diagnosis of Toxoplasma gondii was established on the basis of the optimization of antigenic epitopes of GRA1 and GRA7 genes. Western blot and ELISA results showed that expressed rGRA exhibited specifically reaction with Toxoplasma gondii-positive mouse serum. This strip could be used to diagnose the toxoplasmosis of mouse. Moreover, compared with ELISA and PCR, GICA strip exhibited similar sensitivity in the diagnosis of Toxoplasma gondii in pigs. Here, we preliminarily established a diagnostic method for rapidly detecting toxoplasmosis, which may provide technical support for large-scale screening of Toxoplasma gondii.

中文翻译：

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弓形虫诊断胶体金免疫层析试纸条的制备

摘要 弓形体病作为一种危害严重的人畜共患病，临床症状复杂，病变不典型，难以及时诊断。因此，开发一种合适的技术来诊断和预防弓形体病已成为许多研究的主题。本研究在优化GRA1和GRA7基因抗原表位的基础上,建立了一种用于诊断弓形虫的胶体金免疫层析试纸条。Western印迹和ELISA结果表明表达的rGRA与弓形虫阳性小鼠血清表现出特异性反应。该条可用于诊断小鼠弓形体病。此外，与ELISA和PCR相比，GICA条在猪弓形虫的诊断中表现出相似的敏感性。这里，