ABSTRACT Maximum permissible levels of mycotoxins in baby food may be 1% of those in ordinary food. Therefore, highly sensitive methods of mycotoxin control are in demand. To detect such low amounts, expensive instrumental methods are commonly used. Advantages of immunochromatographic analyses are their low cost and simple sample preparation; however, their sensitivity needs to be increased to contend with instrumental methods. A scheme for competitive immunochromatography with indirect labelling was implemented and developed for the detection of mycotoxin zearalenone (ZEA). Two separate reagents were used for the assay, namely free specific antibodies and antispecies antibodies conjugated with gold nanoparticles. This made it possible to simultaneously increase the sensitivity of the assay and the reliability of measurements. The instrumental detection limit of ZEA in baby food was 5 pg/mL (100 pg/g). Thus, the sensitivity attained is comparable with liquid chromatography characteristics. The duration of the analysis was 17 min.

中文翻译：

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婴儿食品中玉米赤霉烯酮间接标记的高灵敏度横向流动测试

摘要 婴儿食品中霉菌毒素的最大允许含量可能是普通食品的 1%。因此，需要高度灵敏的霉菌毒素控制方法。为了检测如此低的数量，通常使用昂贵的仪器方法。免疫层析分析的优点是成本低，样品制备简单；然而，它们的灵敏度需要提高以应对仪器方法。实施并开发了一种用于检测霉菌毒素玉米赤霉烯酮 (ZEA) 的间接标记竞争免疫层析方案。两种单独的试剂用于该测定，即游离特异性抗体和与金纳米颗粒缀合的反物种抗体。这使得可以同时提高测定的灵敏度和测量的可靠性。婴儿食品中 ZEA 的仪器检测限为 5 pg/mL (100 pg/g)。因此，获得的灵敏度与液相色谱特性相当。分析持续时间为17分钟。