ABSTRACT Flammulina velutipes contains bioactive polysaccharides that can activate the immune system. Here we report the functional characterization in an immunomodulatory F. velutipes polysaccharide (FVP) both in vitro and in vivo. In cell experiment, FVP was discovered to be an activator to induce the M1-polarization of the RAW 264.7 cells. In animal experiment, FVP was found to raise the gene expression of IL-1β, iNOS and ZO-1 in mice colon tissue. Moreover, it seemed to achieve the immune regulative function by raising the relative abundance of Allobaculum, Lactobacillus, Alloprevotella, Akkermansia and Bifidobacterium. Gut microbiota also caused the body’s functional changes, enriched the harmful chemicals’ degradation ability and weakened the metabolism of carbohydrates. These findings suggest that FVP is an immunomodulatory polysaccharide that can boost cellular immune responses and a potential prebiotic agent in regulating gut microbiota.

中文翻译：

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基于肠道微生物群调节的免疫激活金针菇多糖的体外和体内功能表征

摘要金针菇含有可激活免疫系统的生物活性多糖。在这里，我们报告了体外和体内免疫调节 F. velutipes 多糖 (FVP) 的功能表征。在细胞实验中，发现 FVP 是一种激活剂，可诱导 RAW 264.7 细胞的 M1 极化。在动物实验中，发现FVP可以提高小鼠结肠组织中IL-1β、iNOS和ZO-1的基因表达。此外，它似乎通过提高异芽孢杆菌、乳杆菌、异普氏菌、阿克曼菌和双歧杆菌的相对丰度来实现免疫调节功能。肠道菌群还引起机体功能变化，丰富了有害化学物质的降解能力，削弱了碳水化合物的代谢。