ABSTRACT Tricaine (TMS) is usually used as an anesthetic in the transportation of aquatic animals. However, when consumed, the numerous residual drug residues found in fish can cause a high risk to human health. Therefore, it is important to develop a rapid and sensitive method for the detection of this drug in food. Here we have developed a novel lateral-flow immunochromatographic strip (LF-ICS) method for the detection of TMS in fish samples, using a very effective anti-TMS monoclonal antibody with a half-maximal inhibitory concentration value of 31.79 ng/mL. After the K2CO3 and coating antigen concentrations were optimized in phosphate buffered saline (pH 7.4), the LF-ICS was used to determine TMS levels in fish samples spiked with varying concentrations of the drug. Following this, the values for the visual limit of detection and the cut-off for TMS were found to be less than 0.1 and 1 μg/mL, respectively. These results indicated that our developed LF-ICS is applicable for on-site screening of TMS in fish samples.

中文翻译：

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用于快速检测鱼类样品中三卡因的免疫层析试纸条

摘要 Tricaine (TMS) 通常用作水生动物运输中的麻醉剂。然而，当食用时，鱼类中发现的大量残留药物残留会对人类健康造成很高的风险。因此，开发一种快速、灵敏的方法来检测食品中的这种药物非常重要。在这里，我们开发了一种新的横向流动免疫色谱条 (LF-ICS) 方法，用于检测鱼样品中的 TMS，使用非常有效的抗 TMS 单克隆抗体，半数最大抑制浓度值为 31.79 ng/mL。在磷酸盐缓冲盐水 (pH 7.4) 中优化 K2CO3 和涂层抗原浓度后，LF-ICS 用于确定添加不同浓度药物的鱼样品中的 TMS 水平。按照此，发现视觉检测限和 TMS 的临界值分别小于 0.1 和 1 μg/mL。这些结果表明我们开发的 LF-ICS 适用于鱼类样品中 TMS 的现场筛查。