Frontiers in Genetics ( IF 2.8 ) Pub Date : 2020-06-29 , DOI: 10.3389/fgene.2020.00764 Qi Guo 1, 2 , Junjie Wang 1, 2 , Runlu Sun 1, 2 , Zhijian He 1, 2 , Qian Chen 1, 2 , Wenhao Liu 1, 2 , Maoxiong Wu 1, 2 , Jinlan Bao 1, 2 , Zhaoyu Liu 1, 2 , Jingfeng Wang 1, 2 , Yuling Zhang 1, 2
Hypertrophic cardiomyopathy (HCM), the most common heritable cardiomyopathy, is associated with a high risk of sudden cardiac death. The complexity and behavior of the circular RNA (circRNA)-associated competing endogenous RNA (ceRNA) network in HCM have not been thoroughly elucidated. Plasma circRNA and messenger RNA (mRNA) expression profiles were acquired by using a microarray. Weighted correlation network analysis (WGCNA) and linear models for microarray data (Limma) were used to analyze microarray data. Gene modules, consisting of genes with high correlations, were detected and represented by a designated color. The ceRNA network, including circRNA, microRNA (miRNA), and mRNA, was constructed based on the “ceRNA hypothesis” using an integrated systems biology method. By WGCNA, two modules, namely magenta and red modules, were identified as being positively correlated with HCM. In the combined analysis of WGCNA and Limma, 36 hub circRNAs in the magenta module and 83 hub circRNAs in the red module were significantly upregulated compared with the controls. By coexpression analysis, 270 circRNA–mRNA pairs were identified with a coefficient ≥0.9 and
中文翻译:
环状RNA相关竞争内源RNA网络的全面构建通过整合分析鉴定了肥厚性心肌病中的新型环状RNA。
肥厚型心肌病(HCM)是最常见的遗传性心肌病,与心脏猝死的高风险相关。HCM中与环状RNA(circRNA)相关的竞争内源性RNA(ceRNA)网络的复杂性和行为尚未得到充分阐明。通过使用微阵列获得血浆circRNA和信使RNA(mRNA)表达谱。加权相关网络分析(WGCNA)和微阵列数据的线性模型(Limma)用于分析微阵列数据。检测到由具有高度相关性的基因组成的基因模块,并用指定的颜色表示。使用集成系统生物学方法,基于“ ceRNA假设”构建了包括circRNA,microRNA(miRNA)和mRNA在内的ceRNA网络。根据WGCNA,两个模块,即洋红色和红色模块,被确定为与HCM正相关。在WGCNA和Limma的组合分析中,与对照相比,品红色模块中的36个hub circRNA和红色模块中的83个hub circRNA显着上调。通过共表达分析,鉴定出270个circRNA-mRNA对,其系数≥0.9和