Root transcriptomic profile was comparatively studied in a serpentine (TM) and a non-metallicolous (NTM) population of Noccaea goesingensis in order to investigate possible features of Ni hyperaccumulation. Both populations were characterised by contrasting Ni tolerance and accumulation capacity. The growth of the TM population was unaffected by metal excess, while the shoot biomass production in the NTM population was significantly lower in the presence of Ni in the culture medium. Nickel concentration was nearly six- and two-fold higher in the shoots than in the roots of the TM and NTM population, respectively. The comparison of root transcriptomes using the RNA-seq method indicated distinct responses to Ni treatment between tested ecotypes. Among differentially expressed genes, the expression of IRT1 and IRT2, encoding metal transporters, was upregulated in the TM population and downregulated/unchanged in the NTM ecotype. Furthermore, differences were observed among ethylene metabolism and response related genes. In the TM population, the expression of genes including ACS7, ACO5, ERF104 and ERF105 was upregulated, while in the NTM population, expression of these genes remained unchanged, thus suggesting a possible regulatory role of this hormone in Ni hyperaccumulation. The present results could serve as a starting point for further studies concerning the plant mechanisms responsible for Ni tolerance and accumulation.

中文翻译：

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诺卡氏菌的金属和非金属生态型对镍过量的转录组响应。


对Noccaea gosingensis的蛇纹石 (TM) 和非金属 (NTM) 种群的根转录组谱进行了比较研究，以研究 Ni 超积累的可能特征。两个种群的特点是镍耐受性和积累能力不同。 TM群体的生长不受金属过量的影响，而当培养基中存在Ni时，NTM群体的地上部生物量产量显着降低。 TM 和 NTM 种群芽中的镍浓度分别比根中的镍浓度高出近六倍和两倍。使用 RNA-seq 方法比较根转录组表明测试的生态型之间对 Ni 处理的不同反应。在差异表达基因中，编码金属转运蛋白的IRT1和IRT2的表达在TM群体中上调，而在NTM生态型中下调/不变。此外，在乙烯代谢和反应相关基因之间观察到差异。在TM群体中， ACS7 、 ACO5 、 ERF104和ERF105等基因的表达上调，而在NTM群体中，这些基因的表达保持不变，因此表明该激素在Ni超积累中可能具有调节作用。目前的结果可以作为进一步研究负责镍耐受和积累的植物机制的起点。