Millet is a dangerous weed in crop fields. A lack of seed dormancy helps it to spread easily and be present in maize, wheat, and other crop fields. Our previous report revealed the possibility that millet can also play a role as a virus reservoir. In that study, we focused on visual symptoms and detected the presence of several viruses in millet using serological methods, which can only detect the presence of the investigated pathogen. In this current work, we used small RNA high-throughput sequencing as an unbiased virus diagnostic method to uncover presenting viruses in randomly sampled millet grown as a volunteer weed in two maize fields, showing stunting, chlorosis, and striped leaves. Our results confirmed the widespread presence of wheat streak mosaic virus at both locations. Moreover, barley yellow striate mosaic virus and barley virus G, neither of which had been previously described in Hungary, were also identified. As these viruses can cause severe diseases in wheat and other cereals, their presence in a weed implies a potential infection risk. Our study indicates that the presence of millet in fields requires special control to prevent the emergence of new viral diseases in crop fields.

中文翻译：

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小米既可以作为杂草，又可以作为作物田中的病毒库。

小米是农作物中的危险杂草。种子休眠的缺乏帮助其易于传播，并存在于玉米，小麦和其他农作物田地。我们之前的报告揭示了小米还可能充当病毒库的可能性。在该研究中，我们着眼于视觉症状，并使用血清学方法检测了小米中几种病毒的存在，这些方法只能检测所调查病原体的存在。在当前的工作中，我们使用小RNA高通量测序作为无偏病毒诊断方法，以发现在两个玉米田中作为志愿者杂草生长的随机采样的小米中呈现的病毒，显示发育迟缓，萎黄和条纹状叶片。我们的结果证实了小麦条纹花叶病毒在两个地方都广泛存在。此外，大麦黄条纹花叶病毒和大麦病毒G，也没有发现匈牙利以前曾描述过的两种方法。由于这些病毒会在小麦和其他谷物中引起严重的疾病，因此它们存在于杂草中意味着潜在的感染风险。我们的研究表明，小米在田间的存在需要特殊控制，以防止在田间出现新的病毒性疾病。