Rapid and miniaturized method for detection of hygiene indicators, Escherichia coli and coliforms, in dairy products,Journal of Food Safety

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Rapid and miniaturized method for detection of hygiene indicators, Escherichia coli and coliforms, in dairy products
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-07-27 , DOI: 10.1111/jfs.12839
Saurabh Kadyan ₁ , Naresh Kumar ₁ , Ramakant Lawaniya ₁ , Pradeep Kumar Sharma ₁ , Bhawna Arora ₁ , Nimisha Tehri ₁

Affiliation

Current investigation was aimed to develop colorimetric tests for rapid detection of Escherichia coli/coliforms. These test (s) for E. coli and coliforms were developed using the modified E. coli selective medium (M‐ECSM) and coliform selective medium, respectively. The selective media contain a combination of group specific marker enzymes and selective agents. The marker enzymes were screened using chromogenic substrates wherein β‐D‐glucuronidase and glutamate decarboxylase were found specific for E. coli while β‐D‐galactosidase for coliforms. The selectivity of the media was achieved using different concentrations of ampicillin and gentamicin. The optimized test procedures enabled sensitive detection of 0.35 ± 0.10 log cfu/ml of E. coli and 0.57 ± 0.15 log cfu/ml of coliforms at 37°C within 14.30 ± 0.45 and 12.15 ± 0.30 hr, respectively. M‐ECSM selectively inhibited major Enterobacteriaceae contaminants (Salmonella, Shigella, and Yersinia) up to 6 log cfu/ml. Moreover, better selectivity of M‐ECSM was reported against tested commercial chromogenic media. Field evaluation of developed test (s) reported prevalence of E. coli/coliforms as 57.29/88.54% in 96 raw milk and 16.28/51.16% in 43 pasteurized milk samples. Further, test components were vacuum dried in the form of miniaturized point‐of‐need test for field application in dairy farms and industries with minimal infrastructural requirements.

中文翻译：

快速，小型化的乳制品卫生指标，大肠杆菌和大肠菌的检测方法

当前的研究旨在开发用于快速检测大肠杆菌/大肠杆菌的比色法。分别使用改良的大肠杆菌选择培养基（M-ECSM）和大肠菌选择培养基开发了针对大肠杆菌和大肠菌的测试。选择性培养基包含基团特异性标记酶和选择性试剂的组合。使用生色底物筛选标记酶，其中发现β-D-葡萄糖醛酸苷酶和谷氨酸脱羧酶对大肠杆菌具有特异性，而β-D-半乳糖苷酶对大肠菌具有特异性。使用不同浓度的氨苄西林和庆大霉素可实现培养基的选择性。优化的测试程序可实现0.35±0.10 log cfu / ml的灵敏检测大肠杆菌和0.57±0.15 log cfu / ml的大肠菌在37°C下分别在14.30±0.45和12.15±0.30小时内。M‐ECSM选择性抑制主要肠杆菌科细菌污染物（沙门氏菌，志贺氏菌和耶尔森氏菌），最高可达6 log cfu / ml。此外，据报道，相对于经测试的商业发色介质，M-ECSM具有更好的选择性。发达试验的现场评估报告，大肠杆菌/大肠菌的流行率在96种原奶中为57.29 / 88.54％，在43种巴氏灭菌奶样品中为16.28 / 51.16％。此外，测试组件以微型化的现场测试形式真空干燥，可用于奶牛场和基础设施要求最低的行业。

更新日期：2020-07-27

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