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Paths of cucumber green mottle mosaic virus disease spread and disinfectant‐based management
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2020-07-27 , DOI: 10.1111/aab.12629 Elinor Darzi 1, 2 , Oded Lachman 1 , Elisheva Smith 1 , Amnon Koren 3 , Eyal Klein 3 , Nadav Pass 3 , Omer Frenkel 1 , Aviv Dombrovsky 1
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2020-07-27 , DOI: 10.1111/aab.12629 Elinor Darzi 1, 2 , Oded Lachman 1 , Elisheva Smith 1 , Amnon Koren 3 , Eyal Klein 3 , Nadav Pass 3 , Omer Frenkel 1 , Aviv Dombrovsky 1
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Cucumber green mottle mosaic virus (CGMMV) assigned to the genus Tobamovirus is considered a major disease cause of cucurbits worldwide. A primary route for CGMMV disease spread is via mechanical contact. The virus is highly stable and adheres to various agricultural equipment. In the current study, we examined means to inactivate the virus and reduce disease spread via planting equipment and supplies using various chemicals. We have found that incubations of CGMMV‐infected cucumber plant extracts with MENNO‐Florades 2%, Virocid 3% or Green Up D 20% inactivated the virus and prevented disease spread in a biological assay. Stabilised chlorine formulation (KlorBac), which has the active ingredient troclosene‐sodium (sodium dichloroisocyanurate, SDIC) at 2,000 ppm, was efficient in disinfecting CGMMV‐contaminated grafting knives in 2 s. Similarly, immersing virus contaminated grafting knives for 2 s in 20% (wt/vol) non‐fat milk powder reduced infectivity of the contaminated knives. CGMMV‐contaminated nursery sowing trays could constitute a primary infectious viral source transmitted via irrigation water. CGMMV‐contaminated sowing trays immersed in KlorBac 2,000 ppm or active oxygen (Huwa‐San TR‐50) 1%, were efficiently disinfected. Interestingly, hydrophobic insulation of the CGMMV‐contaminated trays using dry silicone layers reduced initiation of the viral primary infection in CGMMV‐contaminated new sowing trays but was less efficient in CGMMV‐contaminated re‐used trays. Importantly, Septadine (0.5% chlorhexidine gluconate) was not effective in disinfection of grafting knives. Notably, CGMMV‐infected cucumber plant extract incubated with 20% (wt/vol) non‐fat milk powder was refractory to the milk suggesting that virus release from surfaces did not necessarily involve virus inactivation.
中文翻译：
黄瓜绿斑驳花叶病毒病的传播途径和基于消毒剂的管理
属于烟草花叶病毒属的黄瓜绿斑驳花叶病毒（CGMMV）被认为是全世界葫芦科病的主要病因。CGMMV疾病传播的主要途径是通过机械接触。该病毒是高度稳定的，可粘附在各种农业设备上。在当前的研究中，我们研究了通过使用各种化学药品的种植设备和供应品灭活病毒并减少疾病传播的方法。我们发现，将CGMMV感染的黄瓜植物提取物与MENNO-Florades 2％，Virocid 3％或Green Up D 20％一起孵育可以使病毒失活，并在生物学检测中防止了疾病传播。稳定的氯配方（KlorBac）具有有效成分troclone-sodium（二氯异氰尿酸钠，SDIC），浓度为2,000 ppm，可在2 s内对CGMMV污染的嫁接刀进行有效消毒。同样，将受病毒污染的嫁接刀在20％（wt / vol）的脱脂奶粉中浸泡2秒钟，可降低受污染刀的传染性。受CGMMV污染的苗圃播种盘可能构成了通过灌溉水传播的主要传染性病毒源。浸泡在2,000 ppm KlorBac或1％活性氧（Huwa-San TR-50）中的CGMMV污染的播种盘已得到有效消毒。有趣的是，使用干燥的有机硅层对CGMMV污染的托盘进行疏水绝缘，可以减少CGMMV污染的新播种托盘中病毒原发感染的发生，但在CGMMV污染的重复使用托盘中效率较低。重要的是，Septadine（0.5％葡萄糖酸洗必泰）对嫁接刀的消毒无效。值得注意的是
更新日期：2020-07-27
中文翻译：
黄瓜绿斑驳花叶病毒病的传播途径和基于消毒剂的管理
属于烟草花叶病毒属的黄瓜绿斑驳花叶病毒（CGMMV）被认为是全世界葫芦科病的主要病因。CGMMV疾病传播的主要途径是通过机械接触。该病毒是高度稳定的，可粘附在各种农业设备上。在当前的研究中，我们研究了通过使用各种化学药品的种植设备和供应品灭活病毒并减少疾病传播的方法。我们发现，将CGMMV感染的黄瓜植物提取物与MENNO-Florades 2％，Virocid 3％或Green Up D 20％一起孵育可以使病毒失活，并在生物学检测中防止了疾病传播。稳定的氯配方（KlorBac）具有有效成分troclone-sodium（二氯异氰尿酸钠，SDIC），浓度为2,000 ppm，可在2 s内对CGMMV污染的嫁接刀进行有效消毒。同样，将受病毒污染的嫁接刀在20％（wt / vol）的脱脂奶粉中浸泡2秒钟，可降低受污染刀的传染性。受CGMMV污染的苗圃播种盘可能构成了通过灌溉水传播的主要传染性病毒源。浸泡在2,000 ppm KlorBac或1％活性氧（Huwa-San TR-50）中的CGMMV污染的播种盘已得到有效消毒。有趣的是，使用干燥的有机硅层对CGMMV污染的托盘进行疏水绝缘，可以减少CGMMV污染的新播种托盘中病毒原发感染的发生，但在CGMMV污染的重复使用托盘中效率较低。重要的是，Septadine（0.5％葡萄糖酸洗必泰）对嫁接刀的消毒无效。值得注意的是
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