The objective of this study was to investigate the effects of tetrandrine (TET) on cell migration and invasion of nasopharyngeal carcinoma NPC‐TW 039 cells in vitro. TET at 1–10 μM did not change cell morphology and also did not decrease the total cell viability and proliferation in NPC‐TW 039 cells. It decreased the cell mobility based on decreased wound closure in NPC‐TW 039 cells by wound healing assay. TET suppressed the cell migration and invasion using transwell system. TET reduced MMP‐2 activities at 1–10 μM and these effects are in dose‐dependently. After exposed to various treatments, TET decreased the levels of p‐ERK, p‐JNK, p‐p38, RhoA, and NF‐κB at 48 hr. Based on these findings, we may suggest TET‐inhibited cell migration and invasion of NPC‐TW 039 cells via the suppression of MAPK and RhoA signaling pathways for inhibiting the MMP‐2 and ‐9 expression in vitro.

中文翻译：

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粉防己碱通过抑制 MAPK 和 RhoA 信号通路抑制人鼻咽癌 NPC-TW 039 细胞的细胞迁移和侵袭。


本研究的目的是探讨粉防己碱（TET）对体外鼻咽癌NPC-TW 039细胞迁移和侵袭的影响。 1-10 μM 的 TET 不会改变细胞形态，也不会降低 NPC-TW 039 细胞的总细胞活力和增殖。通过伤口愈合测定，NPC-TW 039 细胞的伤口闭合减少，从而降低了细胞迁移率。 TET利用transwell系统抑制细胞迁移和侵袭。 TET 在 1-10 μM 时可降低 MMP-2 活性，并且这些作用呈剂量依赖性。接受各种治疗后，TET 在 48 小时时降低了 p-ERK、p-JNK、p-p38、RhoA 和 NF-κB 的水平。基于这些发现，我们可能认为 TET 可以通过抑制 MAPK 和 RhoA 信号通路来抑制体外 MMP-2 和 ‐9 的表达，从而抑制 NPC-TW 039 细胞的细胞迁移和侵袭。