Campylobacter jejuni is an important foodborne pathogen with global distribution. We describe a genotyping study of a collection of C. jejuni (n = 137) isolated from different broiler farms and from multiple sites along the processing line in a slaughterhouse in Argentina during 2011, 2012 and 2015. The isolates were genotyped using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Based on the PFGE results, the isolates were grouped into 26 pulsotypes. Subsequently, the isolates representing these 26 pulsotypes were chosen for MLST genotyping, which identified 16 different sequence types (STs) and 6 clonal complexes (CCs) (21, 45, 48, 353, 354, 446). Several of the STs (n = 7) have not been previously reported in the PubMLST.org database. The most prevalent CCs were 21, 45 (both associated with human campylobacteriosis worldwide) and 353. This study showed high genetic diversity among C. jejuni in the broiler production environment in Argentina with novel MLST genotypes.

空肠弯曲杆菌是一种全球分布的重要食源性病原体。我们描述了2011、2012 和 2015 年间从不同肉鸡养殖场和阿根廷屠宰场加工线上的多个地点分离的空肠弯曲杆菌（n  =  137）的基因分型研究。使用脉冲场对分离株进行基因分型凝胶电泳 (PFGE) 和多位点序列分型 (MLST)。根据 PFGE 结果，将分离株分为 26 个脉冲型。随后，选择代表这 26 种脉冲型的分离株进行 MLST 基因分型，鉴定出 16 种不同的序列类型 (ST) 和 6 种克隆复合体 (CC)（21、45、48、353、354、446）。几个 STs (n  = 7) 以前没有在 PubMLST.org 数据库中报道过。最普遍的 CC 是 21、45（均与世界范围内的人类弯曲杆菌病有关）和 353。这项研究表明，阿根廷肉鸡生产环境中空肠弯曲杆菌的遗传多样性很高，具有新的 MLST 基因型。