A bacterial consortium immobilized in magnetic floating biochar gel beads is proposed to remove high-molecular-weight polycyclic aromatic hydrocarbons. The microbial consortium performed better than single strains and consisted of four strains of marine bacteria for degrading pyrene (PYR), two strains for benzo(a)pyrene (BAP), and three strains for indeno(1,2,3-cd)pyrene (INP), which were isolated from oil-contaminated seawater. The immobilized cells could biodegrade 89.8%, 66.9% and 78.2% of PYR, BAP and INP, respectively, and had better tolerance to pH, temperature and salinity than free cells. The Andrews model was used to explore the biodegradation kinetics, and when the initial concentrations of PYR, BAP, and INP were 7.80, 3.05, and 3.41 mg/L, the specific biodegradation rates reached maximum values of 0.2507, 0.1286, and 0.1930 d⁻¹, respectively. The immobilized microbial consortium had a high HMW-PAH removal ability and good floatability and magnetic properties and could be collected by an external magnetic field.

提出了一种固定在磁性漂浮生物炭凝胶珠上的细菌联合体，以去除高分子量多环芳烃。微生物联盟的表现优于单个菌株，包括用于降解pyr（PYR）的四个海洋细菌菌株，用于苯并（a）re（BAP）的两个菌株和用于茚并（1,2,3- cd）的三个菌株py（INP），是从受石油污染的海水中分离出来的。固定化细胞可分别对PYR，BAP和INP进行89.8％，66.9％和78.2％的生物降解，并且对pH，温度和盐度的耐受性优于游离细胞。安德鲁斯模型用于探索生物降解动力学，并且当PYR，BAP和INP的初始浓度分别为7.80，3.05，和3.41 mg / L时，该特定生物降解率达到0.2507，0.1286和0.1930 d最大值^{- 1}。固定化的微生物财团具有很高的HMW-PAH去除能力，良好的漂浮性和磁性，可以被外部磁场收集。