Canine kobuvirus (CaKoV) is a causative agent of gastroenteritis in dogs. Rapid detection of CaKoV is important for preventing and controlling this condition. In this study, an SYBR Green I-based quantitative real-time PCR assay was established for CaKoV detection. Specific primers targeting a highly conserved region of the CaKoV 3D gene were developed. After optimization, the method detected a minimum of 1 × 10¹ copies/μL with high specificity, stability, and repeatability. Moreover, the entire process only required approximately 1.5 h for completion. Our results were supported by those obtained for clinical samples, in which our developed method was successfully applied. The newly established real-time PCR is a rapid, sensitive, specific, and repeatable method for the quantitative detection of CaKoV and can, therefore, be used in epidemiological studies.

犬科布病毒（CaKoV）是犬胃肠炎的病原体。CaKoV的快速检测对于预防和控制这种情况很重要。在这项研究中，建立了基于SYBR Green I的定量实时PCR分析法用于CaKoV检测。开发了针对CaKoV 3D基因高度保守区域的特异性引物。优化后，该方法检测到的最小值为1×10 ¹拷贝/μL具有高特异性，稳定性和可重复性。此外，整个过程仅需要约1.5小时即可完成。我们的结果得到了临床样品的支持，这些结果在我们的开发方法中得到了成功应用。新建立的实时PCR是一种快速，灵敏，特定且可重复的定量检测CaKoV的方法，因此可以用于流行病学研究。