A multiresidue method for identification and quantification of 45 veterinary antibiotics (VAs) belonging to 5 different drug classes has been developed, which is the first comprehensive method for pig manure. The proposed method used mixed liquor with methanol: acetonitrile: citrate buffer ratio of 1:1:2 to extract the target VAs from freeze-dried manure. The extracting solution passed through solid-phase extraction (SPE) procedure with Oasis HLB cartridges, which could isolate the target VAs and clean the samples in a single step. After blow-drying and concentrating, the samples were analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in one single injection with a run time of 12 min. The average recovery rates for most of the VAs were in the range of 50–120% with relative standard deviations below 15%. Linearity was studied in the range between 2 and 1000 μg/kg using matrix-matched calibration and pure solvent calibration, to evaluate the matrix effects (MEs). The limits of detection (LDs) and quantification (LQs) were in the range of 0.01–1.54 μg/kg and 0.03–5.13 μg/kg respectively, except for penicillin G. Finally, the proposed method was used to quantify VA residues in real swine manure from three farms with different scales; tetracyclines, sulfonamides, quinolones and macrolides were the most frequently detected compounds.

已开发出一种用于鉴定和定量5种不同药物类别的45种兽用抗生素的多残留方法，这是第一种用于猪粪的综合方法。所提出的方法使用混合液与甲醇：乙腈：柠檬酸盐的缓冲液比例为1：1：2，以从冻干肥料中提取目标VA。萃取溶液通过Oasis HLB柱通过固相萃取（SPE）程序，可以分离目标VA并在一个步骤中清洁样品。吹干并浓缩后，通过超高效液相色谱-串联质谱法（UHPLC-MS / MS）在单次进样中分析样品，运行时间为12分钟。大多数VA的平均回收率在50–120％的范围内，相对标准偏差低于15％。使用基质匹配校准和纯溶剂校准研究了线性范围为2至1000μg/ kg的线性，以评估基质效应（MEs）。除了青霉素G外，检出限（LDs）和定量限（LQs）分别在0.01–1.54μg/ kg和0.03–5.13μg/ kg范围内。最后，提出的方法用于定量分析真实的VA残留量来自三个规模不同的农场的猪粪；四环素，磺酰胺，喹诺酮和大环内酯类化合物是最常检测到的化合物。该方法用于量化三个规模不同的猪场中真实猪粪中的VA残留量。四环素，磺酰胺，喹诺酮和大环内酯类化合物是最常检测到的化合物。该方法用于量化三个规模不同的猪场中真实猪粪中的VA残留量。四环素，磺酰胺，喹诺酮和大环内酯类化合物是最常检测到的化合物。