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An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli
Bioorganic & Medicinal Chemistry ( IF 3.3 ) Pub Date : 2020-07-28 , DOI: 10.1016/j.bmc.2020.115662 Claudio Zambaldo 1 , Minseob Koh 1 , Fariborz Nasertorabi 2 , Gye Won Han 2 , Abhishek Chatterjee 3 , Raymond C Stevens 2 , Peter G Schultz 1
中文翻译：
用于大肠杆菌非经典氨基酸诱变的正交seryl-tRNA合成酶/tRNA对
更新日期：2020-08-11
Bioorganic & Medicinal Chemistry ( IF 3.3 ) Pub Date : 2020-07-28 , DOI: 10.1016/j.bmc.2020.115662 Claudio Zambaldo 1 , Minseob Koh 1 , Fariborz Nasertorabi 2 , Gye Won Han 2 , Abhishek Chatterjee 3 , Raymond C Stevens 2 , Peter G Schultz 1
Affiliation
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We report the development of the orthogonal amber-suppressor pair Archaeoglobus fulgidus seryl-tRNA (Af-tRNASer)/Methanosarcina mazei seryl-tRNA synthetase (MmSerRS) in Escherichia coli. Furthermore, the crystal structure of MmSerRS was solved at 1.45 Å resolution, which should enable structure-guided engineering of its active site to genetically encode small, polar noncanonical amino acids (ncAAs).
中文翻译：
用于大肠杆菌非经典氨基酸诱变的正交seryl-tRNA合成酶/tRNA对
我们报告了大肠杆菌中正交琥珀抑制子对Archaeoglobus fulgidus seryl-tRNA (Af-tRNA Ser )/ Methanosarcina mazei seryl-tRNA 合成酶 (MmSerRS) 的发展。此外，MmSerRS 的晶体结构以 1.45 Å 的分辨率解析，这将使其活性位点的结构引导工程能够遗传编码小的极性非规范氨基酸 (ncAAs)。
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