The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile phase. Detection was completed densitometrically at 254 nm. The R_F estimation of berberine and rutin was observed to be 0.67 ± 0.02 and 0.47 ± 0.02, respectively. The developed HPTLC method was validated according to ICH guidelines; the method was specific, linear and accurate and can be used to determine berberine and rutin in marketed herbal preparations. The Tinospora cordifolia plant extract was further evaluated for antioxidant activity using HPTLC, and berberine was found to be more active than rutin during DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. The method was found simple, rapid, accurate, specific and robust for the analysis of berberine and rutin in crude drug using the same method.

Graphical abstract

中文翻译：

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新型HPTLC指纹方法的开发，用于同时估算药用植物及其药物制剂中的小ber碱和芦丁，然后将其应用于抗氧化剂测定

本研究旨在开发和验证高效定量薄层色谱（HPTLC）系统，用于同时定量测定堇菜叶提取物及其药物制剂中的小ber碱和芦丁。层析展开用的共混物进行Ñ（：1.1：1.1：2.5，10己烷，乙酸乙酯，冰醋酸和甲醇体积/体积）作为流动相。在254nm光密度检测完成。在[R _˚F小碱和芦丁的估计值分别为0.67±0.02和0.47±0.02。根据ICH指南验证了开发的HPTLC方法；该方法具有特异性，线性和准确性，可用于测定市售草药制剂中的小ber碱和芦丁。的心叶青牛胆植物提取物用于抗氧化活性用HPTLC进一步评估，和黄连中发现比芦丁多种活性DPPH（2,2-二苯基-1-苦基肼）清除活性期间。发现该方法简单，快速，准确，专一且可靠，可用于使用相同方法分析原料药中的小ber碱和芦丁。

图形概要