The pharmaceutical industry shows an emerging interest in formulas that contain live and beneficial microorganisms, also known as probiotics or pharmabiotics, which in many cases, are host-specific. The resistance to higher temperature is an essential feature of these microorganisms when working on the design of products for vaginal formula. In order to obtain a high number of viable cells and a prolonged shelf life in the designed product, it is required to apply technological procedures using high temperatures or abrupt changes of them, which result in conditions that are different from the optimal growth temperature and can affect the metabolic capabilities of the bacteria when administered to the host in order to reestablish the ecological mucosa. The aim of this work was to evaluate the behavior of 30 different species and strains of autochthonous beneficial vaginal lactobacilli (BVL) when exposed to high temperatures, determine their survival capabilities and analyze their pre-adaptation to those temperatures, in order that they still maintain their viability after technological processes and further conservation. BVL were exhibited to temperatures higher than optimal, with the purpose of evaluating their growth kinetics and parameters. Later, they were exposed to higher temperatures, and then, returned to their optimal, to determine if they were able to grow again. The strains that showed higher resistance were selected, and their viability and beneficial properties studied further. The growth kinetics of strains exposed to higher temperatures showed different patterns, which provided evidence that the thermal adaptation is strain-dependent and is not related to any particular species and/or metabolic group in which the strains were taxonomically classified. The pre-adaptive step allowed the growth of some of the strains, preserving their viability and probiotic properties after the high temperatures were applied. The results shows that BVL can be exposed to high temperatures used in different technological processes that are applied for pharmabiotic formulations, such as spray dried or vacuum rotary evaporation, and/or during the conservation period. The results obtained indicate that some specific BVL strains resist high temperatures and grow afterwards at optimal conditions.

制药行业对包含活的和有益的微生物（也称为益生菌或药物生物）的配方表现出了浓厚的兴趣，这些微生物在许多情况下是特定于宿主的。当设计阴道用配方产品时，对高温的抗性是这些微生物的基本特征。为了在设计产品中获得大量的活细胞并延长保质期，需要使用高温或突然改变温度的工艺程序，从而导致条件不同于最佳生长温度，并且可能当施用于宿主以重建生态粘膜时，它会影响细菌的代谢能力。这项工作的目的是评估暴露于高温下的30种不同物种和品系的本地有益阴道乳杆菌（BVL）的行为，确定其生存能力并分析其对这些温度的预先适应性，以便它们仍能保持经过技术处理和进一步保护后，它们的生存能力。为了评估其生长动力学和参数，BVL的温度高于最佳温度。后来，将它们置于较高的温度下，然后恢复到最佳状态，以确定它们是否能够再次生长。选择表现出较高抗性的菌株，并进一步研究其生存力和有益特性。暴露于高温下的菌株的生长动力学表现出不同的模式，这提供了热适应性依赖于菌株的证据，并且与对菌株进行分类分类的任何特定物种和/或代谢组均不相关。自适应步骤使某些菌株得以生长，在施加高温后仍能保持其活力和益生菌特性。结果表明，BVL可能会暴露于应用于药物制剂（例如喷雾干燥或真空旋转蒸发）的不同工艺过程中和/或在保存期间所使用的高温。获得的结果表明，某些特定的BVL菌株可以抵抗高温，然后在最佳条件下生长。