Abstract

The prevalence of antibiotic-resistant microorganism strains requires us to search for new antibacterial medicines. The study of the antibacterial effect of metal nanoparticles is mostly dedicated to ultradispersed silver and copper powders, while the antimicrobial activity of nickel nanoparticles is under researched. There are a number of studies suggesting a correlation between metal nanoparticles’ antibacterial activity and their physical and chemical properties. The physical and chemical properties of nickel nanoparticles are studied and oxide film ensuring a prolonged effect of the agent is found on the metal surface. It is shown that nickel nanoparticles form large (1145.00 ± 89.60 nm) agglomerations. Individual nanoparticles are as large as 80.51 ± 2.21 nm. The effect of nickel nanoparticles on the Staphylococcus epidermidis and Escherichia coli clinical strains is studied. The pronounced antibacterial effect of metal nanoparticles dependent on their concentration and exposure time is observed. The research of the potential of the microorganism cell ζ-zeta proves the metal nanoparticles’ adhesion on the surface of a microbe cell due to electrostatic stress. The effect of nickel nanoparticles on Gram-negative and Gram-positive microorganisms’ cell metabolism is estimated and the decrease in the saccharolytic activity of E. coli clinical strains, as well as the reduction of S. epidermidis strains able to turn nitrates into nitrites, is shown.

中文翻译：

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具有特定物理性质的镍纳米粒子对植入物相关感染的活性

摘要

抗生素抗性微生物菌株的流行要求我们寻找新的抗菌药物。金属纳米颗粒的抗菌作用研究主要是针对超分散的银和铜粉，而镍纳米颗粒的抗菌活性仍在研究中。有大量研究表明，金属纳米颗粒的抗菌活性与其物理和化学性质之间存在相关性。研究了镍纳米粒子的物理和化学性质，并在金属表面发现了可确保延长试剂作用的氧化膜。结果表明，镍纳米颗粒形成大的（1145.00±89.60 nm）团聚体。单个纳米粒子的尺寸最大为80.51±2.21 nm。镍纳米粒子对合金的影响研究了表皮葡萄球菌和大肠杆菌临床菌株。观察到取决于金属纳米粒子的浓度和暴露时间的明显的抗菌作用。对微生物细胞ζ-zeta潜力的研究证明，由于静电应力，金属纳米粒子在微生物细胞表面的粘附。估计了镍纳米粒子对革兰氏阴性和革兰氏阳性微生物细胞代谢的影响，并降低了大肠杆菌临床菌株的糖酵解活性，并降低了能够将硝酸盐转化为亚硝酸盐的表皮葡萄球菌菌株，显示。