Soybean seed infected with Colletotrichum truncatum is an important source of primary inoculum for anthracnose epidemics. Based on differences in the GAPDH gene sequences of Colletotrichum species, one pair of species-specific primers, CtruncF1/CtruncR1, was designed to accurately detect C. truncatum in soybean seed samples. The primers amplified only a single PCR band of 211 bp from C. truncatum. SYBR Green qPCR using these primers enabled the detection of DNA of the target fungus in inoculated soybean seeds and in naturally infested seeds. The sensitivity of the method was 0.000253 ng/μL of C. truncatum DNA template, with an efficiency of 1.78 and a Ct of 30.09. These species-specific primers may be useful for certification of soybean seeds aimed at avoiding introduction of the pathogen into soybean-producing regions.

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基于 qPCR 的大豆种子中截生炭疽菌检测

感染了截头孢菌的大豆种子是炭疽病流行的主要接种物的重要来源。基于Colletotrichum种GAPDH基因序列的差异，设计一对种特异性引物CtruncF1/CtruncR1来准确检测大豆种子样品中的C. truncatum。引物仅扩增了来自 C. truncatum 的 211 bp 的单个 PCR 带。使用这些引物的 SYBR Green qPCR 能够检测接种的大豆种子和自然感染的种子中目标真菌的 DNA。该方法的灵敏度为0.000253 ng/μL的C. truncatum DNA模板，效率为1.78，Ct为30.09。这些物种特异性引物可用于认证大豆种子，旨在避免将病原体引入大豆产区。