Hydrocarbons originated from automobile oils are significant polluting agents of the soil. Lipase-mediated catalytic degradation of hydrocarbons from this used automobile oil is attempted in the present approach. Lipase was produced from peanut oil industry waste biomass using thermophilic alkaline-tolerant Pseudomonas sp. S1 obtained from sediment of Taptapani hot spring, Odisha. The experiments were designed as per central composite design, which is response surface methodology, to study the effect of the parameters and their interaction on the activity. A quadratic regression was found to be best with the experimental data, R² = 0.899741 and the maximum lipase production of 51.3 IU/g was predicted from peanut oil seed waste biomass at an optimum pH of 8.31, temperature of 50.58 °C, moisture content of 50.8%, and biosurfactant content of 1.051 mg. The significant interaction of pH and moisture content with biosurfactant during lipase production was observed through the contour plots and 3D surface responses. Pseudomonas sp. S1 could degrade 92.6% of the used automobile oil after incubation for 24 h under optimum pH of 8.31 and temperature of 50.58 °C. Degradation of petroleum hydrocarbons by this thermophilic isolate could be effective due to the ability of lipase to utilize the used automobile oils as sole source of carbon and energy.

来自汽车油的碳氢化合物是土壤的重要污染因子。在本方法中，尝试了由这种用过的汽车油中的脂肪酶介导的烃的催化降解。脂肪酶是使用耐高温的耐碱性假单胞菌（Pseudomonas sp）从花生油工业废料中产生的。S1是从奥里萨邦（Odisha）Taptapani温泉的沉积物中获得的。根据中央复合设计（响应面方法）设计实验，以研究参数及其相互作用对活性的影响。实验数据R ^2的二次方回归最佳。 花生油种子废料生物质在最适pH值为8.31，温度为50.58°C，水分含量为50.8％和生物表面活性剂含量为1.051 mg的条件下预测的最大脂肪酶产量为0.899741 = 0.899741，最大脂肪酶产量为51.3 IU / g。通过等高线图和3D表面响应观察到了脂肪酶生产过程中pH和水分含量与生物表面活性剂之间的显着相互作用。假单胞菌属。在最适pH 8.31和温度50.58°C下孵育24 h后，S1可以降解92.6％的废旧汽车油。由于脂肪酶能够利用用过的汽车油作为碳和能源的唯一来源，因此通过这种嗜热分离物降解石油碳氢化合物可能是有效的。