Innovative and bioactive wound dressings prepared by electrospinning mimicking the native structure of the extracellular matrix (ECM) have gained significant interest as an alternative to conventional wound care applications. In this study, bilayered wound dressing material was produced by sequential electrospinning of quaternized poly(4-vinyl pyridine) (upper layer) on the Centella Asiatica (CA) extract containing electrospun poly(D, L-lactide-co-glycolide) (PLGA)/poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) blend membrane (lower layer). Scanning electron microscopy (SEM) was utilized to show a uniform and bead-free fiber structure of electrospun membranes. The average diameter of CA extract containing electrospun PLGA/PHBV blend membrane was calculated 0.471±0.11 µm, whereas the average fiber diameter of electrospun poly(Q-VP) membranes was in the range of 0.460±0.057 µm. Chemical, thermal, mechanical properties, and adsorption capacity of electrospun membranes, as well as the cumulative release of CA from the electrospun PLGA/PHBV membrane, were investigated. Viability, adhesion, and attachment of human fibroblast cells on the electrospun membranes on pre-set days were evaluated by the colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation Assay (MTS assay) and SEM. Results revealed that CA loaded bilayered electrospun wound dressing showed promoted attachment and proliferation of fibroblasts. Hence, it can be concluded that CA extract containing bilayered electrospun wound dressing prepared in this study has a promising potential for wound healing applications.

通过电纺丝模拟细胞外基质（ECM）的天然结构制备的创新性生物活性伤口敷料，已成为替代常规伤口护理应用的重要兴趣。在这项研究中，通过在积雪草上顺序电纺季铵化的聚（4-乙烯基吡啶）（上层）来生产双层伤口敷料（CA）提取物，包含静电纺丝的聚（D，L-丙交酯-乙交酯共聚物）（PLGA）/聚（3-羟基丁酸酯-共3-羟基戊酸酯）（PHBV）共混膜（下层）。利用扫描电子显微镜（SEM）显示出电纺膜的均匀且无珠的纤维结构。含有电纺PLGA / PHBV共混膜的CA萃取物的平均直径经计算为0.471±0.11 µm，而电纺聚Q-VP膜的平均纤维直径为0.460±0.057 µm。研究了电纺膜的化学，热，机械性能和吸附能力，以及CA从电纺PLGA / PHBV膜中的累积释放。生存力，附着力，通过比色CellTiter96®Aqueous One Solution细胞增殖测定（MTS测定）和SEM评估了预设天的人成纤维细胞在电纺膜上的粘附和附着情况。结果表明，CA负载的双层电纺伤口敷料显示成纤维细胞的附着和增殖得到促进。因此，可以得出结论，在这项研究中制备的含有CA提取物的双层电纺伤口敷料具有潜在的伤口愈合应用潜力。