Traditionally, anaerobic digestion (AD) is applied for the simultaneous generation of energy and treatment of various organic wastes. For example, despite orange peels (OP) and seaweed (SW) are highly available for AD, their physicochemical composition can inhibit the microbiome and collapse the process. In the present work, we examined the combination of OP and SW with livestock manure (MN) at a co-digestion process. At first, batch toxicity tests revealed that D-limonene and gallic acid at 0.2% v/v and 2.28 g L⁻¹ respectively solely inhibit methane generation. At batch assays and based on added volatile solids (VS), the mono-digestion of OP and SW had methane yields of 398.4 ± 9.4 and 348.6 ± 10.2 m³ t_VS⁻¹, respectively. Furthermore, continuous mode experiments were conducted in order to examined process performance and bioenergy outcome at the co-digestion of OP, SW, and MN at various organic loading rates and substrate contributions. This work revealed two successful operation strategies when OP and SW were co-digested with manure resulting in methane yields of 264.5 ± 5.2 and 220.5 ± 5.2 m³ t_VS⁻¹, respectively. The results of this work potentially could be applied when dealing with other types of algal biomass.

传统上，厌氧消化（AD）用于同时产生能量和处理各种有机废物。例如，尽管桔皮（OP）和海藻（SW）可以用于AD，但它们的理化成分可以抑制微生物组并破坏该过程。在目前的工作中，我们在共同消化过程中研究了OP和SW与牲畜粪便（MN）的结合。首先，分批毒性试验显示D-柠檬烯和没食子酸分别为0.2％v / v和2.28 g L ^-1只能抑制甲烷的产生。在分批分析中，基于添加的挥发性固体（VS），OP和SW的单消化甲烷产量分别为398.4±9.4和348.6±10.2 m ³  t _VS ^-1， 分别。此外，进行了连续模式实验，以检验在各种有机负载速率和底物作用下OP，SW和MN共同消化时的工艺性能和生物能结果。这项工作揭示了OP和SW与粪肥共同消化时的两种成功的操作策略，甲烷产量分别为264.5±5.2和220.5±5.2 m ³  t _VS ^-1。当处理其他类型的藻类生物质时，这项工作的结果可能会得到应用。