Mesenchymal stem cells (MSC) are a popular candidate in cellular therapy for many diseases. MSCs are well known by their feature of self-renewal and their differentiation potential. NESTIN is a cytoskeletal protein expressed in MSC that functions directly in cell proliferation and differentiation. Here, we demonstrated that adding UltraGRO, a medium supplement, could maintain and partially recover the expression of NESTIN in human umbilical cord derived MSCs (UC-MSCs). Furthermore, the UC-MSCs cultured with UltraGRO showed a better immunomodulation ability in a colitis mouse model compared with those cultured in other types of media. This indicates that the use of novel culture medium benefits the maintenance of NESTIN expression and NESTIN may be one of the vital factors that regulates the performance of MSCs.

中文翻译：

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使用新型培养基在脐带-MSC中恢复和维持NESTIN表达。

间充质干细胞（MSC）在许多疾病的细胞治疗中是一种流行的候选药物。MSC以其自我更新的特征和分化潜能而闻名。NESTIN是在MSC中表达的一种细胞骨架蛋白，直接在细胞增殖和分化中起作用。在这里，我们证明了添加UltraGRO（一种培养基补充剂）可以维持并部分恢复人脐带来源的MSC（UC-MSC）中NESTIN的表达。此外，与在其他类型的培养基中培养的那些相比，用UltraGRO培养的UC-MSC在结肠炎小鼠模型中显示出更好的免疫调节能力。这表明使用新型培养基有利于维持NESTIN 表达和NESTIN可能是调节MSCs性能的重要因素之一。