The important physiological and pathophysiological roles of intestinal human microbiome (HMB) in human health have been emerging, owing to the access to molecular biology techniques. Herein we evaluated, for the first time, the intestinal HMB through direct hybridization approach using n-counter flex DX technology which bypasses the amplification procedure currently applied by other technologies to study the human microbiome. To this purpose, a clinical study was carried out on fecal samples, recruiting both healthy volunteers (N-FOB) and subjects positive for occult blood (P-FOB). A relevant custom panel of 79 16S rRNA target gene was engineered and 32 of them displayed a variation between the two clusters of subjects. Our findings revealed that bacteria belonging to Proteobacteria have higher distribution in P-FOB describing dysbiosis. Similarly, Bacteroidetes and Firmicutes phylum display high distribution in P-FOB. Of interest, the presence of Clostridium difficile that belongs to Firmicutes phylum displayed about 70% of low presence in N-FOB compared to P-FOB subjects. Only one bacterium belonging to the Actinobacteria phylum, the Bifidobacterium bifidum, was present.

由于获得了分子生物学技术，肠道人类微生物组（HMB）在人类健康中的重要生理和病理生理作用已经出现。在这里，我们首次使用n-counter flex DX技术通过直接杂交方法评估了肠道HMB，该技术绕过了其他技术目前用于研究人类微生物组的扩增程序。为此，对粪便样本进行了临床研究，招募了健康志愿者（N-FOB）和潜血阳性的受试者（P-FOB）。对79个16S rRNA靶基因的相关定制面板进行了工程改造，其中32个展示了两组受试者之间的变异。我们的发现表明，属于变形杆菌的细菌在P-FOB中具有较高的分布。同样，拟杆菌和硬毛门在P-FOB中显示高分布。有趣的是，与P-FOB受试者相比，属于硬毛门的艰难梭菌在N-FOB中显示出约70％的低存在。仅存在一种属于放线菌的细菌，即双歧双歧杆菌。