The impacts of Ho and Li (0, 10, 50, 200 mg/L) were tested towards the growth of four basidiomycetous fungal species, their ability to decolorise synthetic dyes (Reactive Green 19, Reactive Orange 16, Reactive Black 5), and produce oxidative enzymes. All species; Agrocybe dura, Skeletocutis biguttulata, Exidia saccharina and Galerina paludosa; grew with and without supplemented Ho or Li. The growth of S. biguttulata was the most tolerant species towards Ho or Li (200 mg/L), whereas the growth of G. paludosa was the most sensitive of the studied species to both 200 mg Ho or Li/L. All fungi oxidized ABTS [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] forming colour zone on plate tests indicating production of lignin modifying laccase enzyme. A. dura and G. paludosa, formed black MnO₂ zone in Mn²⁺ plates, which indicates the production of manganese peroxidase (MnP). A. dura and G. paludosa decolorised Reactive Black 5 indicating the production of versatile peroxide (VP) enzyme. Our study presents two new candidate species able to produce VP. A. dura was capable of decolorising all tested synthetic dyes in the presence of Ho or Li (0–200 mg/L) suggesting that this fungus is a promising species for bioremediation of multi dye-containing wastes.

测试了 Ho 和 Li (0, 10, 50, 200 mg/L) 对四种担子菌类真菌生长的影响、它们对合成染料（活性绿 19、活性橙 16、活性黑 5）的脱色能力，以及产生氧化酶。所有物种；Agrocybe dura , Skeletocutis biguttulata , Exidia saccharina和Galerina paludosa; 在有和没有补充 Ho 或 Li 的情况下生长。S. biguttulata的生长是对 Ho 或 Li (200 mg/L) 最耐受的物种，而G. paludosa的生长是所研究物种中对 200 mg Ho 或 Li/L 最敏感的物种。所有真菌都氧化了 ABTS [2,2'-叠氮基-双（3-乙基苯并噻唑啉-6-磺酸）]，在平板测试中形成颜色区，表明产生了木质素修饰漆酶。A. dura和G. paludosa在 Mn ²⁺板中形成黑色的 MnO ₂带，表明产生了锰过氧化物酶（MnP）。A. dura和G. paludosa 使活性黑 5 脱色，表明产生了多功能过氧化物 (VP) 酶。我们的研究提出了两种能够产生 VP 的新候选物种。硬脑膜 能够在 Ho 或 Li（0-200 毫克/升）存在下使所有测试的合成染料脱色，表明这种真菌是一种有前途的物种，可用于多种含染料废物的生物修复。