The increasing commercial significance of microbial laccase in various fields compels the supply of enzymes in large quantities at an affordable price. Laccase has been produced by a wide variety of wild cultures of bacteria and fungi, the high yielding strains are always desired to meet the increasing demand of laccase. Although various approaches have been proposed for improving the yield of laccase, UV mutagenesis is known as the best method of improving the strains for better yield of microbial metabolites. However, the reports on the use of UV light in strain improvement for laccase production by the use of Enterobacter sp. are scarce and need to be explored fully. For this purpose present study was aimed to improve the laccase production in Enterobacter cloacae through UV mutagenesis. We report enhanced production of laccase in a high yielding strain of E. cloacae isolated from the soap industry waste. UV mutagenesis of a wild strain of E. cloacae resulted in 3.09 folds further improvement in the production. Optimum laccase synthesis was reported at 26 h, of incubation at 30ºC under neutral pH (7.0) conditions. This high yielding mutant strain will have great industrial significance for laccase production.

中文翻译：

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紫外线诱导的诱变提高了阴沟肠杆菌中漆酶的产生

微生物漆酶在各个领域中日益重要的商业意义迫使以可承受的价格大量供应酶。漆酶是由多种细菌和真菌的野生培养物产生的，始终需要高产菌株来满足漆酶不断增长的需求。尽管已经提出了各种方法来提高漆酶的产率，但是已知紫外诱变是改善菌株以更好地获得微生物代谢物的最佳方法。然而，关于使用肠杆菌属细菌在产漆酶的菌株改良中使用紫外线的报道。稀缺，需要充分探索。为此本研究的目的，以改善漆酶的分泌阴沟 肠杆菌通过紫外线诱变。我们报道了从肥皂工业废料中分离出的高产的阴沟肠杆菌中漆酶的产量提高。阴沟肠杆菌野生菌株的紫外线诱变导致产量进一步提高了3.09倍。据报道，在中性pH（7.0）条件下于30ºC孵育26 h，漆酶合成最佳。这种高产突变株对于漆酶的生产将具有重要的工业意义。