In association with human health there is a growing need to develop nanomaterials using biological approach which must be ecofriendly, non-toxic, and cheaper. Therefore, the present research analyses were framed to synthesis and characterize silver nanoparticles from the extracts of seaweed S. wightii. The synthesized silver nanoparticles were subjected to UV-Visible Spectroscopy, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, High Pressure Liquid Chromatography, X-Ray Diffraction and antibacterial activity of agar well diffusion method. The formation of Ag-NPs was confirmed through the presence of an intense absorption peak at 420 nm using UV-Visible spectroscopy. The HPLC profile showed the presence of six secondary metabolites. The FT-IR spectra of biosynthesized AgNp of present biomolecules are amino acids, esters, polysaccharides, phenols, alkanes, chlorophyll, protein amide-I band, protein amide II band, carboxylic group, glycosidic linkage, carbohydrate, and proteins etc. The morphology of biosynthesized AgNps as reported to be spherical in shape was documented by SEM. The nanoparticles are crystalline in structure was confirmed by XRD. The results of antibacterial activity against the gram positive and gram-negative bacteria of human pathogens confirmed and elucidate that the seaweed synthesized nanoparticles could play a profound and reliable role in nano based medicine therapy.

中文翻译：

---

梧桐（Sargassum wightii）银纳米颗粒的生物合成，表征及其对多重耐药人类病原体的抗菌活性

与人类健康相关联，越来越需要使用生物方法开发纳米材料，该方法必须环保，无毒且便宜。因此，本研究的分析框架旨在从海藻S提取物中合成和表征银纳米颗粒。怀特。对合成的银纳米颗粒进行紫外可见光谱，傅立叶变换红外光谱，扫描电子显微镜，高压液相色谱，X射线衍射和琼脂井扩散法的抗菌活性。通过使用紫外可见光谱在420nm处存在强烈的吸收峰，证实了Ag-NP的形成。HPLC图谱表明存在六种次级代谢产物。本生物分子的生物合成的AgNp的FT-IR光谱是氨基酸，酯，多糖，酚，烷烃，叶绿素，蛋白酰胺I带，蛋白酰胺II带，羧基，糖苷键，碳水化合物和蛋白质等。 SEM记录了据报道呈球形的生物合成的AgNps的量。通过XRD证实纳米颗粒在结构上是结晶的。对人类病原体的革兰氏阳性和革兰氏阴性细菌的抗菌活性的结果证实并阐明，海藻合成的纳米颗粒可以在基于纳米的药物治疗中发挥深刻而可靠的作用。