Class Gastropoda is one of the widely studied invertebrates throughout the globe. But, even now most of the species have been identified only by conventional morphology. DNA barcodes have been greatly useful to delimit the cryptic species. In this study, we have analyzed cytochrome c oxidase I (COI) and 18S rRNA gene sequences from 12 specimens belonging to four species to barcode and examine the molecular variation in gastropods of the Vellar estuary mangroves of Tamil Nadu, India. From the results, it is revealed that, among the four genera, Terebralia, Telescopium, and Cerithidea are closely related than genus Nassarius based on the COI and 18S rRNA gene sequences. The neighbour joining trees also confirmed four distinct clades harbouring four genera. As expected, the within species K2P genetic variation is lesser than between species K2P genetic variation in both the genes. An intensive survey is needed to assess the genetic variation of all gastropods in the manmade Vellar estuary mangroves and nearby natural Pichavaram mangroves with additional molecular markers.

中文翻译：

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利用COI和18S rRNA基因对印度Vellar河口红树林中几种腹足纲动物（家庭：ami科和鼻科）进行DNA条形码编码和初步系统发育分析

腹足纲动物是全球范围内广泛研究的无脊椎动物之一。但是，即使到现在，大多数物种也只能通过常规形态来识别。DNA条形码对界定隐性物种非常有用。在这项研究中，我们分析了来自四个物种的12个标本的细胞色素c氧化酶I（COI）和18S rRNA基因序列，并条码分析了印度泰米尔纳德邦Vellar河口红树林腹足动物的分子变异。结果表明，基于COI和18S rRNA ，四个属中的Terebralia，Telescopium和Cerithidea与Nassarius属密切相关。基因序列。邻近的树木也证实了四个不同的进化枝，它们有四个属。不出所料，两个基因中物种内部的K2P遗传变异小于物种之间的K2P遗传变异。需要进行深入调查，以评估人造Vellar河口红树林和附近的自然Pichavaram红树林中所有腹足动物的遗传变异，并带有附加的分子标记。