The present study was made to analyze the antiproliferative effect of crude venom isolated from sea anemone Calliactis tricolor against human cancer cell lines such as Human Neuroblastoma cell (SHSY5Y), Human Lung Cancer cells (A549) and Human Colon Cancer cells (HT-29). The protein profile of venom was performed by Native PAGE and subunit profile was analyzed by SDS-PAGE. The in vitro cytotoxic effect of crude venom against SHSY5Y, A549, HT-29 and Vero cell lines was evaluated by MTT assay method. All the cells exposed to crude venom showed dose-dependent cytotoxic effect with IC₅₀ of 60 µg/ml for both SHSY5Y and A549 cells and 75 µg/ml for HT-29 cells compared to with IC₅₀ of 100 µg/ml for the Vero cell control. The significant decrease in cell viability was observed in SHSY5Y Human Neuroblastoma cells among other cancer cells. The cellular and nuclear morphological observations revealed the loss of cell morphological integrity along with the prominent damage of nucleus in the cell. This was further confirmed by DNA fragmentation assay. Based on the preliminary results, it could be clearly stated that the crude venom of C. tricolor may have a potential anti-cancerous molecules which can be further explored and used as a tool for Neuroblastoma chemotherapy.

中文翻译：

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三色海葵愈伤组织分离出的粗毒对人癌细胞的细胞毒作用

本研究旨在分析从三色海葵Calliactis中分离出的粗毒液对人癌细胞系（SHSY5Y），人肺癌细胞（A549）和人结肠癌细胞（HT-29）的抗增殖作用。。毒液的蛋白谱通过Native PAGE进行，亚基谱通过SDS-PAGE进行分析。在体外粗毒液的针对SHSY5Y，A549，HT-29和Vero细胞系中的细胞毒性作用通过MTT测定方法进行评价。与IC _50相比 ，所有暴露于粗毒液的细胞均表现出剂量依赖性的细胞毒性作用，SHSY5Y和A549细胞的IC ₅₀均为60 µg / ml，HT-29细胞的IC ₅₀为75 µg / ml。 对于Vero细胞对照，浓度为100 µg / ml。在其他癌细胞中，在SHSY5Y人成神经细胞瘤细胞中观察到细胞活力的显着降低。细胞和核的形态学观察揭示了细胞形态完整性的丧失以及细胞核的显着破坏。DNA片段化测定进一步证实了这一点。根据初步的结果，它可以被明确指出的粗毒液C.三色可具有可被进一步探索和用作神经母细胞瘤的化疗工具的潜在的抗-癌分子。