One and two dimensional electrophoresis is a powerful technique for separation of proteins from complex biological sources. These are effective techniques of gene expression analysis at translational level especially for the species of which genome is not yet sequenced. High resolution electrophoresis separation of proteins from complex biological systems is crucial in protein science. Three protein extraction methods, such as Phenol, Multi detergent and TCA acetone were analyzed in the present investigation to find out appropriate protein extraction method amenable to one dimensional (1-D) and two dimensional (2-D) protein profiling of Haloxylonrecurvum and H. salicornicum. Plant species selected for the present investigation are major components of saline and arid ecosystem and valuable source of food additives, nutritional ingredients, herbal medicines and fuel in the region. Phenolic components present in these plants are major hindrances for extraction of pure proteins. High concentrations of good quality proteins represented by clear, intense and more bands/spots in 1-D and 2-D gels were extracted with Phenol extraction method in comparison to TCA acetone and Multi detergent methods. This is possibly thefirst report on extraction and purification of proteins amenable to 1-D and 2-D proteome profiling of Haloxylon species of Indian arid zone, and forms a basis for further comparative proteome analysis of these two contrasting species with respect to salinity and drought tolerant ability for prospects.

中文翻译：

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梭梭属植物一维和二维蛋白质谱的标准化：梭菌属蛋白质组分析的基础

一维和二维电泳是一种从复杂的生物来源中分离蛋白质的强大技术。这些是在翻译水平进行基因表达分析的有效技术，尤其是对于尚未进行基因组测序的物种。从复杂的生物系统中进行蛋白质的高分辨率电泳分离在蛋白质科学中至关重要。在本研究中，分析了三种蛋白质提取方法，例如苯酚，多功能去污剂和TCA丙酮，以找出适合于梭梭和H的一维（1-D）和二维（2-D）蛋白质谱分析的合适蛋白质提取方法。salicornicum。选择用于本次调查的植物物种是盐分和干旱生态系统的主要成分，也是该地区食品添加剂，营养成分，草药和燃料的宝贵来源。这些植物中存在的酚类成分是提取纯蛋白质的主要障碍。与TCA丙酮和多功能去污剂方法相比，采用苯酚提取法提取了高浓度的高质量蛋白质，这些蛋白质在1-D和2-D凝胶中具有清晰，强烈和更多的条带/斑点。这可能是有关Haloxylon的1-D和2-D蛋白质组图谱分析的蛋白质的提取和纯化的首次报道 印度干旱区的物种，并为进一步对比这两个相反物种的盐度和耐旱性前景的蛋白质组分析奠定了基础。