This paper deals with the validation of a method for the determination of iron in spectrophotometric method in aqueous medium. The method is based on complex formation of iron with thioglycolic acid in alkaline medium in presence of a masking agent to produce a red purple chelate that has an absorption maximum at 535 nm wavelength. Beer-Lambert law is obeyed and linear calibration curves are obtained for the concentration range of iron from 0.1 mg/L to 30 mg/L. The reaction is found to be spontaneous in alkaline medium. The limit of detection and limit of quantification for the developed method are 0.0108 and 0.0345, respectively. Effect of different parameters like molar ratio of iron to different reagents and interferences, effect of time and effect of temperature of this method of determination have been studied. It is found that this method is moderately sensitive and has been successfully applied for the determination of iron(III) in different fields like ceramic materials, clay, sand, glass, stone, soil, water, and any inorganic iron containing compound or alloys. A comparison report is made for Chevron gas field waste material and Certified Reference Material of iron, which is done by atomic absorption and UV-visible spectroscopy techniques and found to be comparable.

中文翻译：

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水介质中分光光度法测定铁的方法验证

本文探讨了在水介质中分光光度法测定铁的方法的验证。该方法基于铁与巯基乙酸在掩蔽剂存在下在碱性介质中的复杂形成，以产生在535 nm波长处具有最大吸收的红色紫色螯合物。遵守Beer-Lambert定律并获得铁浓度范围从0.1 mg / L到30 mg / L的线性校准曲线。发现该反应在碱性介质中是自发的。所开发方法的检出限和定量限分别为0.0108和0.0345。研究了铁与不同试剂的摩尔比和干扰物等不同参数的影响，时间的影响和温度的影响。结果发现，该方法灵敏度中等，已成功用于测定陶瓷，粘土，沙子，玻璃，石材，土壤，水和任何无机含铁化合物或合金等不同领域中的铁（III）。对雪佛龙气田废料和铁的认证参比材料进行了比较报告，该报告是通过原子吸收和紫外可见光谱技术完成的，具有可比性。