Estrogens and estrogen mimics prevalent in aquatic environment are of great environmental concern because of their endocrine disrupting and carcinogenic activities. Looking to the wide variety of natural as well as structurally different synthetic estrogen mimics, a reliable in-vitro assay is required for screening the estrogenic activity of endocrine disrupting compounds (EDCs). Surface plasmon resonance (SPR) is one of the most promising analytical tools to monitor the high-performance biomolecular interactions in a label free, real time format. Present paper demonstrates a facile SPR based affinity bioassay employing estrogen receptor-α, human (hERα) functionalized self assembled monolayer covalently bound onto the gold sensor chip as recognition species. A successful interaction of potential estrogen mimics with estrogen receptor is evidenced by net rise in SPR angle. The assay has been validated in terms of optimum experimental conditions and specificity with estrogen as a positive control showing maximum estrogenic activity. As a proof of concept, proposed affinity assay is tested for screening the estrogenic activity of progesterone, pregnenolone, tamoxifen, and bisphenol-A as representative examples of potential EDCs of different classes.

中文翻译：

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无标记生物传感器，用于筛选雌激素活性

在水生环境中普遍存在的雌激素和雌激素模拟物由于其内分泌干扰和致癌活性而引起了极大的环境关注。期待各种各样的天然和结构不同的合成雌激素模拟物，一种可靠的体外需要进行化验才能筛选内分泌干扰化合物（EDC）的雌激素活性。表面等离振子共振（SPR）是最有前途的分析工具之一，以无标签的实时格式监视高性能的生物分子相互作用。本论文展示了一种简便的基于SPR的亲和生物测定法，该测定法利用共价结合到金传感器芯片上的雌激素受体-α，人（hERα）功能化自组装单层作为识别物质。潜在的雌激素模拟物与雌激素受体的成功相互作用通过SPR角的净上升来证明。该方法已在最佳实验条件和特异性方面进行了验证，其中雌激素作为显示最大雌激素活性的阳性对照。作为概念证明，